Using satellite tobacco mosaic virus vectors for gene silencing
| Autor: | Michael Metzlaff, Veronique Gossele |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
viruses
Nicotiana tabacum Genetic Vectors Biology Microbiology Virus Viral vector Virology Tobacco Tobacco mosaic virus Gene silencing Gene Silencing Tobacco mosaic satellite virus Plant Diseases fungi food and beverages Gene targeting General Medicine biology.organism_classification Plants Genetically Modified Plant Leaves Tobacco Mosaic Virus RNA silencing Phenotype Genetic Techniques Gene Targeting RNA Viral Parasitology Satellite tobacco mosaic virus Helper Viruses |
| Zdroj: | Current Protocols in Microbiology |
| ISSN: | 1934-8533 |
| Popis: | This unit describes the use of satellite tobacco mosaic virus (STMV) vectors in combination with native TMV particles for inducing transient gene silencing in tobacco plants. Target gene fragment selection and insertion, virus delivery procedures, and phenotype screening of silenced plants are described in detail. All critical parameters for tobacco plant cultivation, virus infection, and RNA silencing efficiency are discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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