The site of estradiol sensitization of the gonadotrope is prior to calcium mobilization
| Autor: | P. Michael Conn, Donald E. Smith |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
medicine.medical_specialty
Ionophore chemistry.chemical_element Stimulation Gonadotropin-releasing hormone Calcium Gonadotropic cell General Biochemistry Genetics and Molecular Biology Gonadotropin-Releasing Hormone chemistry.chemical_compound Internal medicine medicine Animals Castration General Pharmacology Toxicology and Pharmaceutics Calcimycin Cells Cultured Veratridine Estradiol Chemistry General Medicine Luteinizing Hormone Rats Endocrinology Pituitary Gland Ovariectomized rat Female Luteinizing hormone |
| Zdroj: | Life Sciences. 30:1495-1498 |
| ISSN: | 0024-3205 |
| Popis: | In primary pituitary cell cultures prepared from ovariectomized rats, estradiol-17B (E 2 ) sensitizes gonadotropes to stimulation of luteinizing hormone (LH) release by gonadotropin releasing hormone (GnRH). The calcium ionophore A23187, which stimulates LH release from the cells by Ca 2+ mobilization at a post-receptor locus, and veratridine, which stimulates LH release by activation of endogenous ion channels, were used to localize the site of E 2 action. Cells cultured in medium which was charcoal stripped (to remove steroids) or which contained 10 −8 M added E 2 responded equally well to the ionophore and equally well to veratridine, indicating that the molecular locus of E 2 action precedes Ca 2+ mobilization. This type of analysis can be used to locate the site of action of compounds which alter the responsiveness of the pituitary to GnRH. |
| Databáze: | OpenAIRE |
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