Immobilization of Glucose Oxidase on Modified-Carbon-Paste-Electrodes for Microfuel Cell
| Autor: | Akhiruddin Maddu, Rini Kurniasih, Inda Setyawati, Laksmi Ambarsari, Popi Asri Kurniatin |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Nanotechnology biosensor 03 medical and health sciences chemistry.chemical_compound Polyaniline Glucose oxidase carbon-paste-electrode glucose oxidase glutaraldehyde nano-fiber polyaniline QD1-999 biology General Chemistry Interfacial polymerization Carbon paste electrode Chemistry 030104 developmental biology chemistry Nanofiber Electrode biology.protein Glutaraldehyde Biosensor Nuclear chemistry |
| Zdroj: | Indonesian Journal of Chemistry, Vol 16, Iss 1, Pp 92-97 (2016) Indonesian Journal of Chemistry; Vol 16, No 1 (2016); 92-97 |
| ISSN: | 2460-1578 1411-9420 |
| DOI: | 10.22146/ijc.21183 |
| Popis: | Glucose oxidase (GOx) is being developed for many applications such as an implantable fuel cell, due to its attractive property of operating under physiological conditions. This study reports the functional immobilization of glucose oxidase onto polyaniline-nanofiber-modified-carbon-paste-electrodes (GOx/MCPE) as bioanodes in fuel cell applications. In particular, GOx is immobilized onto the electrode surface via a linker molecule (glutaraldehyde). Polyaniline, synthesized by the interfacial polymerization method, produces a morphological form of nanofibers (100-120 nm) which have good conductivity. The performance of the polyaniline-modified-carbon-paste-electrode (MCPE) was better than the carbon- paste-electrode (CPE) alone. The optimal pH and temperature of the GOx/MCPE were 4.5 (in 100 mM acetate buffer) and 65 °C, respectively. The GOx/MCPE exhibit high catalytic performances (activation energy 16.4 kJ mol-1), have a high affinity for glucose (Km value 37.79 µM) and can have a maximum current (Imax) of 3.95 mA. The sensitivity of the bioelectrode also was high at 57.79 mA mM-1 cm-2. |
| Databáze: | OpenAIRE |
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