Generation of two induced pluripotent stem cell (iPSC) lines from p.F508del Cystic Fibrosis patients
| Autor: | Trond Aasen, Aarne Fleischer, Fernando Gómez, Víctor J. Asensio, Juan Carlos Izpisúa-Belmonte, Iván M. Lorenzo, Víctor Miguel Gálvez, Miguel Servera, Esther Palomino, Daniel Bachiller |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Cell type Cystic Fibrosis células madre pluripotentes inducidas humanos Induced Pluripotent Stem Cells Biology medicine.disease_cause Cystic fibrosis Cell Line 03 medical and health sciences Kruppel-Like Factor 4 SOX2 medicine Animals Humans Induced pluripotent stem cell mutación lcsh:QH301-705.5 Mutation fungi línea celular Cell Biology General Medicine fibrosis quística medicine.disease 3. Good health 030104 developmental biology lcsh:Biology (General) Cell culture KLF4 embryonic structures Cancer research animales Female biological phenomena cell phenomena and immunity Reprogramming Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 29, Iss, Pp 1-5 (2018) Stem Cell Research |
| ISSN: | 1876-7753 |
| Popis: | Cystic Fibrosis (CF) is a monogenic, lethal disease caused by mutations in the cystic fibrosis transmembrane conductance (CFTR) gene. Here we report the production of CF-iPS cell lines from two different p. F508del homozygous female patients (Table 1). Two different primary cell types, skin fibroblasts and keratinocytes, were transfected with retroviral cocktails containing four: c-MYC, KLF4, OCT4 and SOX2 (MKOS) or three: KLF4, OCT4 and SOX2 (KOS) reprogramming factors. Two fibroblast-derived MKOS lines are described in the main text. The lines carry the p. F508del mutation, have a normal karyotype, express pluripotency markers and are able to differentiate into the three germ layers. |
| Databáze: | OpenAIRE |
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