Furin Enzyme and pH Synergistically Triggered Aggregation of Gold Nanoparticles for Activated Photoacoustic Imaging and Photothermal Therapy of Tumors
| Autor: | Mingyuan Gao, Y Eugene Chin, Jingwei Xu, Jianan Ding, Haibin Shi, Xiaju Cheng, Xiuxia Zhou, Zhifang Chai, Huawei Xia, Rui Sun |
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| Rok vydání: | 2021 |
| Předmět: |
Photothermal Therapy
Metal Nanoparticles Nanoparticle 010402 general chemistry 01 natural sciences Analytical Chemistry Photoacoustic Techniques In vivo Cell Line Tumor Neoplasms Tumor Microenvironment Humans Furin Tumor microenvironment biology Chemistry 010401 analytical chemistry Hydrogen-Ion Concentration Phototherapy Photothermal therapy In vitro 0104 chemical sciences Colloidal gold Biophysics biology.protein Nanoparticles Gold |
| Zdroj: | Analytical Chemistry. 93:9277-9285 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.1c01713 |
| Popis: | Specific and effective accumulation of nanoparticles within tumors is highly crucial for precise cancer diagnosis and treatment. Therefore, spatiotemporally manipulating the aggregation of small gold nanoparticles (AuNPs) in a tumor microenvironment is of great significance for enhancing the diagnostic and therapeutic efficacy of tumors. Herein, we reported a novel furin enzyme/acidic pH synergistically triggered small AuNP aggregation strategy for activating the photoacoustic (PA) imaging and photothermal (PTT) functions of AuNPs in vivo. Smart gold nanoparticles decorated with furin-cleavable RVRR (Arg-Val-Arg-Arg) peptides (Au-RRVR) were rationally designed and fabricated. Both in vitro and in vivo experiments demonstrated that such Au-RRVR nanoparticles could be simultaneously induced by furin and acidic pH to form large aggregates within tumorous tissue resulting in improved tumor accumulation and retention, which can further activate the PA and PTT effect of AuNPs for sensitive imaging and efficient therapy of tumors. Thus, we believe that this dual-stimuli-responsive aggregation system may offer a universal platform for effective cancer diagnosis and treatment. |
| Databáze: | OpenAIRE |
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