Effects of external low intensity focused ultrasound on electrophysiological changes in vivo in a rodent model of common peroneal nerve injury
| Autor: | Aira Agrawal, Clif Burdette, Andrea Liss, Damian S. Shin, Paul Neubauer, Goutam Ghoshal, Abigail Hellman, Kanakaharini Byraju, Jiang Qian, Yunseo Linda Park, Julia W. Nalwalk, Julie G. Pilitsis, Emery Williams, Tarun Prabhala, Teresa Maietta |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Rodentia Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Dorsal root ganglion Peripheral Nerve Injuries In vivo Ganglia Spinal Sensory threshold medicine Animals Hot plate test business.industry General Neuroscience Peroneal Nerve Rats Electrophysiology 030104 developmental biology medicine.anatomical_structure Hyperalgesia Anesthesia Neuropathic pain business 030217 neurology & neurosurgery Common peroneal nerve Sensory nerve |
| Zdroj: | Neuroscience. 429:264-272 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/j.neuroscience.2020.01.016 |
| Popis: | Non-invasive treatment methods for neuropathic pain are lacking. We assess how modulatory low intensity focused ultrasound (liFUS) at the L5 dorsal root ganglion (DRG) affects behavioral responses and sensory nerve action potentials (SNAPs) in a common peroneal nerve injury (CPNI) model. Rats were assessed for mechanical and thermal responses using Von Frey filaments (VFF) and the hot plate test (HPT) following CPNI surgery. Testing was repeated 24 h after liFUS treatment. Significant increases in mechanical and thermal sensory thresholds were seen post-liFUS treatment, indicating a reduction in sensitivity to pain (p 0.0001, p = 0.02, respectively). Animals who received CPNI surgery had significant increases in SNAP latencies compared to sham CPNI surgery animals (p = 0.0003) before liFUS treatment. LiFUS induced significant reductions in SNAP latency in both CPNI liFUS and sham CPNI liFUS cohorts, for up to 35 min post treatment. No changes were seen in SNAP amplitude and there was no evidence of neuronal degeneration 24 h after liFUS treatment, showing that liFUS did not damage the tissue being modulated. This is the first in vivo study of the impact of liFUS on peripheral nerve electrophysiology in a model of chronic pain. This study demonstrates the effects of liFUS on peripheral nerve electrophysiology in vivo. We found that external liFUS treatment results in transient decreased latency in common peroneal nerve (CPN) sensory nerve action potentials (SNAPs) with no change in signal amplitude. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect