Development of Terahertz Time-Domain Spectroscopy for Properties Analysis of Highly Diluted Antibodies
| Autor: | Eugeny E. Fesenko, Nikita V. Penkov |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Materials science
Analytical chemistry 02 engineering and technology 010402 general chemistry lcsh:Technology 01 natural sciences lcsh:Chemistry Molecule General Materials Science solution structure Fourier transform infrared spectroscopy Terahertz time-domain spectroscopy Spectroscopy lcsh:QH301-705.5 Instrumentation Fluid Flow and Transfer Processes Aqueous solution lcsh:T Process Chemistry and Technology Intermolecular force General Engineering 021001 nanoscience & nanotechnology lcsh:QC1-999 dynamics of water molecules 0104 chemical sciences Computer Science Applications Terahertz spectroscopy and technology high dilutions lcsh:Biology (General) lcsh:QD1-999 lcsh:TA1-2040 Terahertz spectroscopy THz-TDS Intramolecular force lcsh:Engineering (General). Civil engineering (General) 0210 nano-technology lcsh:Physics |
| Zdroj: | Applied Sciences Volume 10 Issue 21 Applied Sciences, Vol 10, Iss 7736, p 7736 (2020) |
| ISSN: | 2076-3417 |
| Popis: | This work represents the development of a three-band (Debye relaxation, intermolecular bending, and stretching vibrational bands) approach to the terahertz time-domain spectroscopy (THz-TDS) for the analysis of aqueous solutions of lactose saturated with highly diluted (HD) antibodies to interferon-gamma. Since THz-TDS is sensitive to a very slight changes in water properties, we aimed to assess its applicability for the analysis of HD antibodies. To evaluate water features in tested samples we also used Fourier transform infrared spectroscopy, considering the first overtone of intramolecular stretching vibrations of water molecules. We showed a broadening of the energy distribution of intermolecular bonds in the solution of HD substances in comparison to the control solution. This broadening corresponded to the presence of an active component even in HD samples. The results of both methods were similar however, with the THz-TDS it was possible to analyze the changes in energy distribution of intermolecular (hydrogen) bonds in the solutions. Therefore, our approach to THz-TDS method is applicable for analyzing structural properties of the solutions containing HD antibodies and for the indirect investigation of altered aqueous properties in water solutions when direct measurements of a substance concentration are not available. |
| Databáze: | OpenAIRE |
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