Aflibercept Intervention in Experimental Branch Retinal Vein Occlusion Results in Upregulation of DnaJ Homolog Subfamily C Member 17
| Autor: | Danson V Muttuvelu, Alexander Nørgård Alsing, Anders Kruse, Jonas Ellegaard Nielsen, Svend Kirkeby, Benn Falch Sejergaard, Shona Pedersen, Henrik Vorum, Bent Honoré, Lasse Jørgensen Cehofski |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Article Subject genetic structures Pharmacology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation Heat shock protein medicine Macular edema Aflibercept business.industry RE1-994 medicine.disease Fold change eye diseases Vascular endothelial growth factor Blot Ophthalmology 030104 developmental biology chemistry 030221 ophthalmology & optometry Branch retinal vein occlusion sense organs business medicine.drug Research Article |
| Zdroj: | Cehofski, L J, Kruse, A, Alsing, A N, Sejergaard, B F, Nielsen, J E, Pedersen, S, Muttuvelu, D V, Kirkeby, S, Honoré, B & Vorum, H 2021, ' Aflibercept Intervention in Experimental Branch Retinal Vein Occlusion Results in Upregulation of DnaJ Homolog Subfamily C Member 17 ', Journal of Ophthalmology, vol. 2021, 6690260 . https://doi.org/10.1155/2021/6690260 Journal of Ophthalmology Cehofski, L J, Kruse, A, Alsing, A N, Sejergaard, B F, Nielsen, J E, Pedersen, S, Muttuvelu Danson, V, Kirkeby, S, Honoré, B & Vorum, H 2021, ' Aflibercept intervention in experimental branch retinal vein occlusion results in up regulation of DnaJ homolog subfamily C member 17 ', Journal of Ophthalmology . Journal of Ophthalmology, Vol 2021 (2021) |
| Popis: | Aflibercept is an inhibitor of vascular endothelial growth factor (VEGF) used to treat macular edema following branch retinal vein occlusion (BRVO). Despite well-documented efficacy, there is limited knowledge about proteome changes following aflibercept intervention in BRVO. Proteome changes may provide insights into mechanisms of action as well as aspects related to safety profile. In seven Danish Landrace pigs, BRVO was induced with a well-established experimental model of argon laser-induced BRVO. BRVO was induced in both eyes. Three days after the induced BRVO, aflibercept was injected intravitreally in the right eyes, while the left eyes received intravitreal isotonic saline water. Retinas were collected 15 days after the induced BRVO and analyzed with label-free quantification liquid chromatography tandem mass spectrometry (LFQ LC-MS/MS). Fourteen proteins were changed in expression following aflibercept intervention in the BRVO model. LFQ LC-MS/MS identified an upregulation of DnaJ homolog subfamily C member 17 (DNAJC17) (fold change = 6.19) and a modest downregulation of isoform 2 of the protein encoded by N-myc downstream regulated gene 2 (NDRG2) (fold change = 0.40). NDRG2 was unchanged by Western blotting. In the additional significantly regulated proteins, only discrete changes were observed (fold changes 0.52–1.59). Our study is the first to report an association between aflibercept intervention and the heat shock protein DNAJC17. Our results indicate that the role of heat shock proteins in the treatment of BRVO should be further explored. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|