Immunological crossreactivity between a cloned antigen of Onchocerca volvulus and a component of the retinal pigment epithelium
| Autor: | G. Braun, F. Engelbrecht, Clare Gilbert, Vivienne Connor, James A. G. Whitworth, N M McKechnie, D. W. Taylor |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
Male
Pathology medicine.medical_specialty Molecular Sequence Data Immunology Fluorescent Antibody Technique Helminth genetics Cross Reactions Biology Retina Epitope Antigen Onchocerciasis Ocular medicine Animals Humans Immunology and Allergy Amino Acid Sequence RNA Messenger Onchocerca Antigens Cloning Molecular Pigment Epithelium of Eye Gene Library Antiserum Retinal pigment epithelium Base Sequence Articles DNA biology.organism_classification Molecular biology Onchocerca volvulus Recombinant Proteins eye diseases Molecular Weight medicine.anatomical_structure Antigens Helminth Protein Biosynthesis Cattle Female sense organs |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 0022-1007 |
| Popis: | Onchocerciasis (river blindness) is a major blinding disease in Africa, Central America, and South America. Loss of vision can be due to corneal change, optic atrophy, or chorioretinal disease. It has been suggested that autoimmunological reactions resulting from crossreactivity between parasite antigens and components of eye tissues contribute to development of ocular pathology. Using sera collected from onchocerciasis patients as a screening reagent, a cDNA clone (Ov39) has been isolated from a lambda gt11 expression library of Onchocerca volvulus. This antigen exhibits immunological crossreactivity with a component of retinal pigment epithelium cells (RPE). Antiserum raised against this recombinant peptide immunoprecipitates a 22,000 Mr antigen of adult O. volvulus and recognizes a 44,000 Mr component of bovine RPE by Western blotting. A 44,000 Mr antigen of cultured human RPE metabolically labeled with 35S-methionine can be immunoprecipitated with the same antiserum. An antigen of the same size is recognized by a rabbit antiserum raised against whole O. volvulus extract. Immunocytochemical studies on cryostat sections of the bovine eye using the antirecombinant sera localizes this antigen to the RPE. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|