Chlamydia trachomatis recombinant MOMP encapsulated in PLGA nanoparticles triggers primarily T helper 1 cellular and antibody immune responses in mice: a desirable candidate nanovaccine
| Autor: | Praseetha Subbarayan, Saurabh Dixit, Alain B Waffo, Shree R. Singh, Murtada Taha, Abebayehu N. Yilma, Stacie J Fairley, Chino D Cambridge, Vida A. Dennis |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_treatment
Pharmaceutical Science Chlamydia trachomatis medicine.disease_cause chemistry.chemical_compound Mice 0302 clinical medicine Polylactic Acid-Polyglycolic Acid Copolymer International Journal of Nanomedicine vaccine antibody Drug Discovery bacteria Original Research 0303 health sciences Mice Inbred BALB C Immunogenicity Antibody titer General Medicine Flow Cytometry Antibodies Bacterial Recombinant Proteins 3. Good health PLGA medicine.anatomical_structure Bacterial Vaccines Vaccines Subunit Female Antibody Chemokines Adjuvant T cell Biophysics Porins Bioengineering macromolecular substances Biology Cell Line Biomaterials 03 medical and health sciences Immune system medicine Animals Lactic Acid 030304 developmental biology Macrophages Organic Chemistry technology industry and agriculture Th1 Cells Molecular biology cytokines chemistry PLGA nanoparticles biology.protein Nanoparticles Polyglycolic Acid 030215 immunology |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 1176-9114 |
| Popis: | Stacie J Fairley, Shree R Singh, Abebayehu N Yilma, Alain B Waffo, Praseetha Subbarayan, Saurabh Dixit, Murtada A Taha, Chino D Cambridge, Vida A Dennis Center for NanoBiotechnology Research, Alabama State University, Montgomery, AL, USA Abstract: We recently demonstrated by in vitro experiments that PLGA (poly D, L-lactide-co-glycolide) potentiates T helper 1 (Th1) immune responses induced by a peptide derived from the recombinant major outer membrane protein (rMOMP) of Chlamydia trachomatis, and may be a promising vaccine delivery system. Herein we evaluated the immune-potentiating potential of PLGA by encapsulating the full-length rMOMP (PLGA-rMOMP), characterizing it in vitro, and investigating its immunogenicity in vivo. Our hypothesis was that PLGA-rMOMP triggers Th1 immune responses in mice, which are desirable prerequisites for a C. trachomatis candidate nanovaccine. Physical-structural characterizations of PLGA-rMOMP revealed its size (approximately 272 nm), zeta potential (−14.30 mV), apparent spherical smooth morphology, and continuous slow release pattern. PLGA potentiated the ability of encapsulated rMOMP to trigger production of cytokines and chemokines by mouse J774 macrophages. Flow cytometric analyses revealed that spleen cells from BALB/c mice immunized with PLGA-rMOMP had elevated numbers of CD4+ and CD8+ T cell subsets, and secreted more rMOMP-specific interferon-gamma (Th1) and interleukin (IL)-12p40 (Th1/Th17) than IL-4 and IL-10 (Th2) cytokines. PLGA-rMOMP-immunized mice produced higher serum immunoglobulin (Ig)G and IgG2a (Th1) than IgG1 (Th2) rMOMP-specific antibodies. Notably, sera from PLGA-rMOMP-immunized mice had a 64-fold higher Th1 than Th2 antibody titer, whereas mice immunized with rMOMP in Freund's adjuvant had only a four-fold higher Th1 than Th2 antibody titer, suggesting primarily induction of a Th1 antibody response in PLGA-rMOMP-immunized mice. Our data underscore PLGA as an effective delivery system for a C. trachomatis vaccine. The capacity of PLGA-rMOMP to trigger primarily Th1 immune responses in mice promotes it as a highly desirable candidate nanovaccine against C. trachomatis. Keywords: Chlamydia trachomatis, bacteria, vaccine, antibody, cytokines, PLGA nanoparticles |
| Databáze: | OpenAIRE |
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