Chronic low dose-rate radiation down-regulates transcription related to mitosis and chromosomal movement similar to acute high dose in prostate cells
| Autor: | Giannoula Klement, J. Tyson McDonald, Ingolf A. Tuerk, Douglas Schneider, Aleksandr Perepletchikov, Christine Briggs, Michael J. Peluso, Lynn Hlatky, Heather Szelag |
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| Rok vydání: | 2014 |
| Předmět: |
DNA Replication
Male Pathology medicine.medical_specialty Cell Survival Down-Regulation Mitosis Biology Chromosomes Ionizing radiation Biological pathway Prostate health services administration Radiation Ionizing Gene expression medicine Humans Radiology Nuclear Medicine and imaging Cell Proliferation Oligonucleotide Array Sequence Analysis Recombination Genetic Radiological and Ultrasound Technology Gene Expression Profiling Cell Cycle Dose-Response Relationship Radiation Cell cycle Fibroblasts humanities Gene expression profiling Dose–response relationship medicine.anatomical_structure Cancer research Algorithms Genome-Wide Association Study |
| Zdroj: | International journal of radiation biology. 90(3) |
| ISSN: | 1362-3095 |
| Popis: | Despite concerns over risks from exposure to low-dose ionizing radiations encountered in the environment and workplace, the molecular consequences of these exposures, particularly at representative doses and dose-rates, remains poorly understood.Using a novel flood source construct, we performed a direct comparison of genome-wide gene expression regulations resulting from exposure of primary human prostate fibroblast cultures to acute (10 cGy and 200 cGy) and longer-term chronic (1.0-2.45 cGy cumulative over 24 h) exposures.Expression profiling showed significant differential regulation of 396 genes with no measureable changes in the acute 10 cGy dose. However, there were 106 genes in common between samples given an acute 200 cGy dose compared to those given chronic doses, most of which were decreased and related to cell cycle or chromosomal movement in M-phase. Biological pathway analysis showed decreases in cell cycle, chromosomal movement, cell survival and DNA replication, recombination and repair as well as a predicted activation of transcriptional regulators TP53, RB1 and CDKN2A. In agreement with these results, prostate epithelial cells given 200 cGy or chronic doses displayed functional decreases in proliferation and mitotic cells.In summary, we showed a contrast to the common observation of constant or reduced effect per unit dose as the dose (acute) was diminished, that even very low total doses delivered chronically could rival the perturbing effect of acute doses 100 times as intense. Underscored is the importance of the means of dose delivery, shown to be as important as dose size when considering biologic effect. |
| Databáze: | OpenAIRE |
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