Autologous Cardiomyocyte Transplantation in an Ovine Myocardial Infarction Model
| Autor: | J. J. Park, Wongon Kim, C. Y. Na, Doo Hyun Chung |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Cell Transplantation Myocardial Infarction 030232 urology & nephrology Biomedical Engineering Medicine (miscellaneous) Bioengineering 030204 cardiovascular system & hematology Biomaterials Gross examination Infundibulum 03 medical and health sciences 0302 clinical medicine Fibrosis Internal medicine Animals Medicine Myocardial infarction Heart Failure Sheep business.industry Myocardium General Medicine medicine.disease Transplantation medicine.anatomical_structure Heart failure Models Animal Cardiology Ligation business Artery |
| Zdroj: | The International Journal of Artificial Organs. 25:61-66 |
| ISSN: | 1724-6040 0391-3988 |
| DOI: | 10.1177/039139880202500110 |
| Popis: | Background To evaluate the possibility of autologous cardiomyocyte transplantation, we transplanted cultured autologous cells into an infarct region developed by coronary artery ligation in an ovine model. Materials and Methods A chronic heart failure model with a considerable portion of myocardial infarction was created in sheep using sequential ligation of the homonymous artery and its diagonal branch. Autologous cardiomyocytes were cultured and isolated from the right ventricular infundibulum. After a predetermined interval (one animal for two months and the other for three months), the two animals were reanesthetized and a suspension of cultured autologous vetricular cells in 0.3 ml of culture medium (1.2 × 107 cells) was injected into the center of three out of the four sites in the infarcted area using a tuberculin syringe. The same amount of culture medium was injected with an identical procedure into the center of the remaining site, as control. The animals were kept alive for a further month, and then sacrificed for postmortem heart examinations. Light microscopic analysis and immunohistochemical study for myoglobin were done. Results and Conclusions On postmortem gross examination, well-demarcated thin-walled anteroseptal infarcts with chamber enlargement were clearly seen in specimens from the two animals. Microscopic analysis showed homogenous fibrosis throughout the infarcted areas. In both animals, one of the three sites of cardiomycyte injection showed an islet of cardiomyocytes in the mid-myocardium, while none were observed in the control site of either animal. A layer of cardiomyocytes was observed in subendocardial regions, as it was in the control areas. In conclusion, cardiomyocyte transplantation into the infarct regions developed by coronary artery ligation in an ovine model was achieved with only limited success. An understanding of why only 33% of cardiomyocyte-injection sites demonstrated viable cardiomyocytes, in the form of tiny cell islets, remains to be elucidated. |
| Databáze: | OpenAIRE |
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