High temperature induces apoptosis and oxidative stress in pufferfish (Takifugu obscurus) blood cells
| Autor: | Yu-Tao Miao, Xiao-Yan Chen, Chang-Hong Cheng, Fang-Fang Yang, An-Li Wang, Shaoan Liao, Jia-Wen Tan, Chaoxia Ye |
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| Rok vydání: | 2015 |
| Předmět: |
Fish Proteins
Physiology Apoptosis Caspase 3 Biology medicine.disease_cause Biochemistry Superoxide dismutase Heat shock protein medicine Animals Heat shock Heat-Shock Proteins chemistry.chemical_classification Glutathione Peroxidase Reactive oxygen species Blood Cells Superoxide Dismutase Catalase Molecular biology Takifugu Hsp70 Oxidative Stress chemistry biology.protein Calcium Apoptosis Regulatory Proteins Reactive Oxygen Species General Agricultural and Biological Sciences Heat-Shock Response Oxidative stress Developmental Biology |
| Zdroj: | Journal of Thermal Biology. 53:172-179 |
| ISSN: | 0306-4565 |
| DOI: | 10.1016/j.jtherbio.2015.08.002 |
| Popis: | Water temperature is an important environmental factor in aquaculture farming that affects the survival and growth of organisms. The change in culture water temperature may not only modify various chemical and biological processes but also affect the status of fish populations. In previous studies, high temperature induced apoptosis and oxidative stress. However, the precise mechanism and the pathways that are activated in fish are still unclear. In the present study, we investigated the effects of high temperature (34°C) on the induction of apoptosis and oxidative stress in pufferfish (Takifugu obscurus) blood cells. The data showed that high temperature exposure increased oxygen species (ROS), cytoplasmic free-Ca(2+) concentration and cell apoptosis. To test the apoptotic pathway, the expression pattern of some key apoptotic related genes including P53, Bax, caspase 9 and caspase 3 were examined. The results showed that acute high temperature stress induced up-regulation of these genes, suggesting that the p53-Bax pathway and the caspase-dependent apoptotic pathway could be involved in apoptosis induced by high temperature stress. Furthermore, the gene expression of antioxidant enzymes (Cu/Zn-SOD, Mn-SOD, CAT, GPx, and GR) and heat shock proteins (HSP90 and HSP70) in the blood cells were induced by high temperature stress. Taken together, our results showed that high temperature-induced oxidative stress may cause pufferfish blood cells apoptosis, and cooperatively activated p53-Bax and caspase-dependent apoptotic pathway. |
| Databáze: | OpenAIRE |
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