Role of the nuclear membrane protein Emerin in front-rear polarity of the nucleus
| Autor: | Dimos Poulikakos, Alexander A. Mironov, Paulina Nastały, Galina V. Beznoussenko, Divya Purushothaman, Tobias Lendenmann, Orso Maria Romano, Aldo Ferrari, Marco Cosentino Lagomarsino, Stefano Marchesi, Gururaj Rao Kidiyoor, Stefania Lavore, Paolo Maiuri, Alessandro Poli |
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| Přispěvatelé: | Nastaly, P, Purushothaman, D, Marchesi, S, Poli, A, Lendenmann, T, Kidiyoor, Gr, Beznoussenko, Gv, Lavore, S, Romano, Om, Poulikakos, D, Lagomarsino, Mc, Mironov, Aa, Ferrari, A, Maiuri, P |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Nuclear Envelope Polarity (physics) Science Blotting Western Emerin Fluorescent Antibody Technique General Physics and Astronomy Article General Biochemistry Genetics and Molecular Biology Cell Line Myoblasts 03 medical and health sciences 0302 clinical medicine Microscopy Electron Transmission Cell polarity medicine Humans Cell migration Nuclear membrane Nuclear protein Cytoskeleton lcsh:Science Cell Nucleus Microscopy Confocal Multidisciplinary Chemistry Endoplasmic reticulum digestive oral and skin physiology Membrane Proteins Nuclear Proteins General Chemistry Cell biology 030104 developmental biology medicine.anatomical_structure RNA Interference lcsh:Q Nucleus 030217 neurology & neurosurgery |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020) Nature Communications, 11 (1) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.3929/ethz-b-000414003 |
| Popis: | Cell polarity refers to the intrinsic asymmetry of cells, including the orientation of the cytoskeleton. It affects cell shape and structure as well as the distribution of proteins and organelles. In migratory cells, front-rear polarity is essential and dictates movement direction. While the link between the cytoskeleton and nucleus is well-studied, we aim to investigate if front-rear polarity can be transmitted to the nucleus. We show that the knock-down of emerin, an integral protein of the nuclear envelope, abolishes preferential localization of several nuclear proteins. We propose that the frontally biased localization of the endoplasmic reticulum, through which emerin reaches the nuclear envelope, is sufficient to generate its observed bias. In primary emerin-deficient myoblasts, its expression partially rescues the polarity of the nucleus. Our results demonstrate that front-rear cell polarity is transmitted to the nucleus and that emerin is an important determinant of nuclear polarity. Nature Communications, 11 (1) ISSN:2041-1723 |
| Databáze: | OpenAIRE |
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