Antioxidant status and hormonal profile reflected by experimental feeding of probiotics
| Autor: | Magdy A. Ghoneim, Said S Moselhy |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Blood Glucose
0301 basic medicine medicine.medical_specialty Antioxidant Health Toxicology and Mutagenesis medicine.medical_treatment Administration Oral DNA Fragmentation Biology Toxicology Antioxidants Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound Internal medicine medicine Animals chemistry.chemical_classification Triiodothyronine Probiotics Glutathione peroxidase Insulin Public Health Environmental and Occupational Health Glutathione Malondialdehyde Hormones 030104 developmental biology Endocrinology chemistry biology.protein Rabbits Oxidoreductases Hormone |
| Zdroj: | Toxicology and Industrial Health. 32:741-750 |
| ISSN: | 1477-0393 0748-2337 |
| DOI: | 10.1177/0748233713506768 |
| Popis: | Excessive production of free radicals can result in tissue damage, which mainly involves generation of hydroxyl radical and other oxidants. Such free radical-induced cell damage appears to play a major role in the pathogenesis of many diseases. Probiotics have been used therapeutically to modulate immunity, improve digestive processes, lower cholesterol, treat rheumatoid arthritis, and prevent cancer. The proposed research was designed to evaluate the changes in oxidative and antioxidative profile in addition to metabolic-related hormones of living animal model, which may generally affect the health status. Two groups of rabbits (10 animals each) were allocated in hygienic cages of controlled animal house. Control group received standard diet, and the other group received the same diet containing one probiotic for 30 days. Lactate dehydrogenase (LDH) activity in leukocytes, blood glucose, reduced glutathione (GSH), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) were estimated in different tissues. Malondialdehyde (MDA) and total proteins were also determined in different tissues. Certain hormones related to metabolism and growth were also evaluated. Leukocytic LDH activity was significantly increased along with nonsignificant increase of blood glucose in probiotics-fed animals. Results showed significant decreases in the levels of triiodothyronine and thyroid-stimulating hormone but showed significant elevations in thyroxine, insulin, growth hormone, and testosterone levels in animals fed with probiotics. Total proteins content was highly significantly elevated in liver, kidneys, and muscles of probiotic-administered animals. Microsomal GSH level was significantly decreased only in skeletal muscles of probiotic-treated animals. MDA was significantly lowered in animal tissues fed with probiotics. GSH-Px activity was elevated in hepatic and muscular microsomes of probiotic-supplemented animals while it was nonsignificantly increased in renal microsomes. Microsomal SOD activity was elevated in liver, kidneys, and skeletal muscles of probiotics-administrated animals. It is concluded that supplementation of probiotic may enhance antioxidant efficacy and scavenge free radicals and thus may be used as a preventive measure for protection against free radicals-induced disorders. |
| Databáze: | OpenAIRE |
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