Rapid nodal staging of head and neck cancer surgical specimens with flow cytometric analysis

Autor: Åsa Kågedal, Ola Winqvist, Lars-Olaf Cardell, Cecilia Drakskog, Gregori Margolin, Susanna Kumlien Georén, Pedro Farrajota Neves da Silva, Valtteri Häyry, Eric Hjalmarsson, Eva Munck-Wikland
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
squamous cell carcinoma
Male
Cancer Research
Pathology
medicine.medical_treatment
Cell Separation
chemistry.chemical_compound
0302 clinical medicine
Coloring Agents
Lymph node
medicine.diagnostic_test
lymph node metastasis
Epithelial cell adhesion molecule
Middle Aged
Epithelial Cell Adhesion Molecule
Immunohistochemistry
Tongue Neoplasms
medicine.anatomical_structure
Oncology
Cervical lymph nodes
Head and Neck Neoplasms
030220 oncology & carcinogenesis
Lymphatic Metastasis
Carcinoma
Squamous Cell
Neck Dissection
Female
Lymph
Sentinel Lymph Node
Adult
medicine.medical_specialty
Proof of Concept Study
Sensitivity and Specificity
Flow cytometry
03 medical and health sciences
medicine
Carcinoma
Humans
Molecular Diagnostics
Aged
Neoplasm Staging
business.industry
flow cytometry
cancer staging
Keratin-8
Mucin-1
Neck dissection
medicine.disease
030104 developmental biology
chemistry
Keratin-5
Histopathology
business
Zdroj: British Journal of Cancer
ISSN: 1532-1827
0007-0920
Popis: Background: Detection of metastatic spread of head and neck cancer to cervical lymph nodes is essential for optimal design of therapy. Undetected metastases lead to mortality, which can be prevented by better detection methods. Methods: We analysed 41 lymph nodes from 19 patients with oral squamous cell carcinoma (OSCC). Each lymph node was divided in two, one half processed for histopathology and the other half dissociated into single-cell suspension, stained for the carcinoma cell markers cytokeratin 5/8 (CK5/8), epithelial cell adhesion molecule (EpCAM) and epithelial mucin (MUC-1), and analysed with flow cytometry. Flow cytometry data were compared with histopathology performed on serial sections and immunohistochemistry. Six cervical lymph nodes from cancer-free patients were used to establish baseline levels in flow cytometry. Results: Flow cytometry analysis (fluorescence-activated cell sorting; FACS) detected all six metastases confirmed by histopathology as well as the histologically negative nodes. Importantly, among nine sentinel lymph nodes, FACS analysis detected
Databáze: OpenAIRE
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