Additional file 1 of 3D-printed microplate inserts for long term high-resolution imaging of live brain organoids

Autor: Mansilla, Mariana Oksdath, Salazar-Hernandez, Camilo, Perrin, Sally L., Scheer, Kaitlin G., Cildir, Gökhan, Toubia, John, Kristyna Sedivakova, Tea, Melinda N., Sakthi Lenin, Ponthier, Elise, Yeo, Erica C. F., Tergaonkar, Vinay, Poonnoose, Santosh, Ormsby, Rebecca J., Pitson, Stuart M., Brown, Michael P., Ebert, Lisa M., Gomez, Guillermo A.
Rok vydání: 2021
DOI: 10.6084/m9.figshare.14355700
Popis: Additional file 1: Supplementary Figure 1 (related to Fig. 2). Representative sections of cerebral organoids grown on the micro-well inserts and stained with Hoechst 33342 and against cleaved caspase 3. Cleaved caspase 3 is observed in the periphery of cortical structures within organoids, without significant differences across the different experimental conditions (i.e. with or without microplate inserts). Panels were made with cropped images (same size for all time points and conditions). Image’s intensity levels were contracted (same extent for each of the channels across all conditions) from their original 16-bit range to 8-bit RGB for Figure preparation in Adobe Illustrator. Scale Bar = 100 μm.
Databáze: OpenAIRE