Calmodulin-binding transcription activator 1 mediates auxin signaling and responds to stresses in Arabidopsis
| Autor: | Orli Snir, Roni Aloni, Dikla Nachmias, Ester Feldmesser, Joy M. Boyce, Marc R. Knight, Sarah Scrase-Field, Nicolas Bouché, Hillel Fromm, Yael Galon |
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| Přispěvatelé: | Faculty of Life Sciences, Department of Plant Sciences, Tel Aviv University [Tel Aviv], Bioinformatics Unit, Department of Biological Services, Weizmann Institute of Science, Department of Plant of Sciences, University of Oxford [Oxford], Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Durham University, Weizmann Institute of Science [Rehovot, Israël] |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
0106 biological sciences
promoter regions genetic indoleacetic acids metabolism Arabidopsis arabidopsis physiology GUS reporter system Plant Science métalobisme arabidopsis metabolism 01 natural sciences signal transduction physiology génétique [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics 03 medical and health sciences Calmodulin-Binding Transcription Activator 1 stress physiological expression de gène Auxin Gene Expression Regulation Plant Stress Physiological calcium-binding proteins genetics Genetics heterocyclic compounds Promoter Regions Genetic gene expression regulation plant 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Indoleacetic Acids Activator (genetics) dna primers fungi Calcium-Binding Proteins food and beverages biology.organism_classification Transport inhibitor Molecular biology base sequence Cell biology reverse transcriptase polymerase chain reaction chemistry Plant hormone calcium-binding proteins physiology Polar auxin transport 010606 plant biology & botany Signal Transduction |
| Zdroj: | Planta Planta, Springer Verlag, 2010, 232 (1), pp.165-178. ⟨10.1007/s00425-010-1153-6⟩ |
| ISSN: | 0032-0935 1432-2048 |
| DOI: | 10.1007/s00425-010-1153-6⟩ |
| Popis: | Auxin is a key plant hormone that regulates various aspects of plant development. However, the mechanisms integrating auxin growth effects with stress responses are not fully understood. In this study, we investigated the possible role of calmodulin-binding transcription activator 1 (CAMTA1), an Arabidopsis thaliana calcium/calmodulin-binding transcription activator, in auxin signaling and its responses to different stresses. Plants harboring the AtCAMTA1 promoter fused to the GUS reporter gene revealed cell-specific expression patterns reminiscent of auxin responses. The responsiveness of CAMTA1 to auxin was further assessed by chemical disturbances in polar auxin transport, and by RT-PCR analysis of gene expression of dissected leaf sections from plants exposed to the auxin transport inhibitor NPA. Furthermore, the intensity and cell-specific expression patterns of CAMTA1 changed significantly and differentially on exposure to increasing salt concentrations and heat. Transcriptome analysis of a camta1 T-DNA insertion mutant revealed 63 up-regulated genes, of which 17 are associated with auxin signaling. Finally, analysis of hypocotyl elongation in the presence and absence of auxin revealed that camta1 T-DNA insertion mutants and CAMTA1-repressor lines are hyper-responsive to auxin compared to wild-type seedlings. Thus, CAMTA1 participates in auxin signaling and responds to abiotic stresses. |
| Databáze: | OpenAIRE |
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