Degradation and fermentation of alpha-gluco-oligosaccharides by bacterial strains from human colon: in vitro and in vivo studies in gnotobiotic rats
| Autor: | Françoise Popot, Zakia Djouzi, S. Somarriba, Vincent Pascal Pelenc, Pierre Monsan, Claude Andrieux, F. Paul, Odette Szylit |
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| Rok vydání: | 1995 |
| Předmět: |
Male
Time Factors Colon ved/biology.organism_classification_rank.species Carboxylic Acids Oligosaccharides Applied Microbiology and Biotechnology Microbiology Feces In vivo Animals Bacteroides Germ-Free Life Clostridium butyricum Clostridium Bifidobacterium breve biology Bacteria ved/biology Short-chain fatty acid Actinomycetaceae Hydrogen-Ion Concentration biology.organism_classification Rats Inbred F344 Diet Rats Kinetics Biochemistry Fermentation Digestion Bifidobacterium Bacteroides thetaiotaomicron |
| Zdroj: | The Journal of applied bacteriology. 79(2) |
| ISSN: | 0021-8847 |
| Popis: | Z. DJOUZI, C. ANDRIEUX, V. PELENC, S. SOMARRIBA, F. POPOT, F. PAUL, P. MONSAN AND O. SZYLIT. 1995. The ability of several human gut bacteria to break down α-1,2 and α-1,6 glycosidic linkages in α-gluco-oligosaccharides (GOS) was investigated in vitro in substrate utilization tests. Bacteroides thetaiotaomicron, Bifidobacterium breve and Clostridium butyricum, which are usually found in the infant gut and have been associated with both beneficial and deleterious effects on health, were studied. α-Gluco-oligosaccharide degradation was compared in vitro and in vivo in gnotobiotic rats associated with these organisms, inoculated alone or in combination. Oligomer breakdown and short chain fatty acid and gas production indicated hydrolysis and fermentation of the substrate. In vitro and in vivo, Cl. butyricum was the least efficient in utilizing GOS, whereas Bact. thetaiotaomicron was the most efficient. Kinetic studies on GOS hydrolysis in pH-regulated fermenters showed that α-1,2 glucosidic bonds, which characterize the substrate, were more resistant than α-1,6 linkages. Adaptation of gnotobiotic rats to a diet containing 2% (w/w) GOS significantly increased the hydrolysis of α-1,2 glucosidic bonds. Combination of bacteria in trixenic rats improved GOS degradation and inhibited Cl. butyricum metabolism. This inhibition was confirmed in pH-regulated fermenters containing GOS as the principal carbon source. The association of beneficial bacteria and GOS may therefore have a potential health-promoting effect in human neonates. |
| Databáze: | OpenAIRE |
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