Processing bodies accumulate in human cytomegalovirus-infected cells and do not affect viral replication at high multiplicity of infection
| Autor: | Eri Seto, Teruki Inoue, Masanobu Yamada, Yoko Nakatani, Hiroki Isomura |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Human cytomegalovirus
Translation viruses Cytomegalovirus P-body Biology Cycloheximide Antibodies Viral Virus Replication chemistry.chemical_compound Stress granule Virology Translation initiation medicine Humans Eukaryotic initiation factor Cells Cultured MRNA decay Messenger RNA RNA virus diseases Translation (biology) Fibroblasts biochemical phenomena metabolism and nutrition medicine.disease Molecular biology Viral replication chemistry Cytoplasm DNA Viral |
| Zdroj: | Virology. :151-161 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2014.04.022 |
| Popis: | Translationally silenced mRNAs are recruited to two major classes of RNA granules in the cytoplasm, processing bodies (PBs) and stress granules (SGs). We show that PBs accumulated after human cytomegalovirus (HCMV) infection. PB assembly after HCMV infection was also detected in the presence of the protein synthesis inhibitor, cycloheximide, but required active RNA synthesis. UV-inactivated HCMV virions were sufficient to induce PB accumulation in HFF cells treated with cycloheximide. Viral IE1 RNA did not colocalize with PBs, and we could not detect an effect of PB accumulation on viral growth. These results may indicate that HCMV inhibits the colocalization of IE1 mRNA with PBs, preventing IE1 mRNA decay and translational inhibition. |
| Databáze: | OpenAIRE |
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