Evaluation of the Role of Oestrogen Contamination in Functional Studies with Uterine RNA from Oestradiol-Treated Rats
| Autor: | N. Dupont-Mairesse, Paul Galand |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class Uterus Biology Tritium Endometrium Ribonucleases Endocrinology Internal medicine medicine Animals Endocrine system Ribonuclease Estradiol Ovary RNA Biological activity Uterine horns Stimulation Chemical Rats medicine.anatomical_structure Estrogen Vagina biology.protein Female Hormone |
| Zdroj: | Endocrinology. 90:936-940 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-90-4-936 |
| Popis: | Numerous studies have shown that uterine RNA obtained from oestradiol stimulated rats (U-RNA) reproduces the effects of oestradiol on the uterine epithelium of spayed rats. The present work was aimed at clarifying whether oestrogen contamination of the RNA preparations could be responsible for the hormone- like effects. U-RNA and vaginal RNA were prepared from rats treated with 10 μg of oestradiol-17β-6.7-3H. These labelled RNA preparations were then assayed for oestrogen contamination and for their biological activity in the uterine horn of spayed rats. The following observations were made: 1. U-RNA and vaginal RNA preparations were contaminated with tritium label. Assuming that this is all in the form of oestradiol, U-RNA would contain 5.10-7 μg oestradiol/100 μg RNA, and vaginal RNA 6.10-7 μg/100 μg RNA. 2. In biological tests, U-RNA only was active in stimulating hypertrophy of the luminal epithelial cells. This activity was lost after treatment of the U-RNA with ribonuclease. 3. Intraluminal administ... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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