Assessing tumor angiogenesis in colorectal cancer by quantitative contrast-enhanced endoscopic ultrasound and molecular and immunohistochemical analysis
| Autor: | Valeriu Surlin, Elena-Tatiana Cartana, Dragoş-Ovidiu Alexandru, Claudia-Valentina Georgescu, Mihai Ioana, Irina F. Cherciu, Dan Ionuţ Gheonea, Gabriel Gruionu, Ioana Streaţă, Elena-Raluca Nicoli, Adrian Saftoiu, Daniel Pirici, Constantin-Daniel Uscatu |
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| Rok vydání: | 2018 |
| Předmět: |
CD31
Oncology Endoscopic ultrasound medicine.medical_specialty Colorectal cancer Angiogenesis Colonoscopy colorectal cancer 03 medical and health sciences 0302 clinical medicine Internal medicine Biopsy medicine Radiology Nuclear Medicine and imaging Hepatology medicine.diagnostic_test business.industry microvascular density Gastroenterology Area under the curve Endoglin medicine.disease digestive system diseases contrast-enhanced endoscopic ultrasound 030220 oncology & carcinogenesis Original Article 030211 gastroenterology & hepatology business vascular endothelial growth factor receptor |
| Zdroj: | Endoscopic Ultrasound |
| ISSN: | 2303-9027 |
| DOI: | 10.4103/eus.eus_7_17 |
| Popis: | Background and Objectives: Data on contrast-enhanced endoscopic ultrasound (CE-EUS) for colorectal cancer (CRC) evaluation are scarce. Therefore, we aimed to assess the vascular perfusion pattern in CRC by quantitative CE-EUS and compare it to immunohistochemical and genetic markers of angiogenesis. Patients and Methods: We performed a retrospective analysis of CE-EUS examinations of 42 CRC patients, before any therapy. CE-EUS movies were processed using a dedicated software. Ten parameters were automatically generated from the time-intensity curve (TIC) analysis: peak enhancement (PE), rise time (RT), mean transit time, time to peak (TTP), wash-in area under the curve (WiAUC), wash-in rate (WiR), wash-in perfusion index (WiPI), wash-out AUC (WoAUC), and wash-in and wash-out AUC (WiWoAUC). The expression levels of the vascular endothelial growth factor receptor 1 (VEGFR1) and VEGFR2 genes were assessed from biopsy samples harvested during colonoscopy. Microvascular density and vascular area were calculated after CD31 and CD105 immunostaining. Results: Forty-two CE-EUS video sequences were analyzed. We found positive correlations between the parameters PE, WiAUC, WiR, WiPI, WoAUC, WiWoAUC, and N staging (Spearman r = 0.437, r = 0.336, r = 0.462, r = 0.437, r = 0.358, and r = 0.378, respectively, P < 0.05), and also between RT and TTP and CD31 vascular area (r = 0.415, and r = 0.421, respectively, P < 0.05). VEGFR1 and VEGFR2 expression did not correlate with any of the TIC parameters. Conclusions: CE-EUS with TIC analysis enables minimally invasive assessment of CRC angiogenesis and may provide information regarding the lymph nodes invasion. However, further studies are needed for defining its role in the evaluation of CRC patients. |
| Databáze: | OpenAIRE |
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