Evidence for functional interaction of plasma membrane electron transport, voltage-dependent anion channel and volume-regulated anion channel in frog aorta
| Autor: | Rashmi P. Rao, J. Prakasa Rao |
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| Rok vydání: | 2011 |
| Předmět: |
Anions
Voltage-dependent anion channel Ranidae In Vitro Techniques General Biochemistry Genetics and Molecular Biology Ion Channels Electron Transport chemistry.chemical_compound Hypotonic Stress medicine Animals NADH NADPH Oxidoreductases Ferricyanides Homocysteine Aorta Enzyme Assays biology Cell Membrane General Medicine Electron transport chain Membrane chemistry Biochemistry Hypotonic Solutions DIDS biology.protein Tonicity Verapamil Ferricyanide Endothelium Vascular General Agricultural and Biological Sciences 4-Chloromercuribenzenesulfonate Oxidation-Reduction medicine.drug |
| Zdroj: | Journal of biosciences. 35(4) |
| ISSN: | 0973-7138 |
| Popis: | Frog aortic tissue exhibits plasma membrane electron transport (PMET) owing to its ability to reduce ferricyanide even in the presence of mitochondrial poisons, such as cyanide and azide. Exposure to hypotonic solution (108 mOsmol/kg H2O) enhanced the reduction of ferricyanide in excised aortic tissue of frog. Increment in ferricyanide reductase activity was also brought about by the presence of homocysteine (100 microM dissolved in isotonic frog Ringer solution), a redox active compound and a potent modulator of PMET. Two plasma-membrane-bound channels, the volume-regulated anion channel (VRAC) and the voltage-dependent anion channel (VDAC), are involved in the response to hypotonic stress. The presence of VRAC and VDAC antagonists-tamoxifen, glibenclamide, fluoxetine and verapamil, and 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS), respectively-inhibited this enhanced activity brought about by either hypotonic stress or homocysteine. The blockers do not affect the ferricyanide reductase activity under isotonic conditions. Taken together, these findings indicate a functional interaction of the three plasma membrane proteins, namely, ferricyanide reductase (PMET), VDAC and VRAC. |
| Databáze: | OpenAIRE |
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