Serotonin in the Pathogenesis of Lymphocytic Colitis
| Autor: | Anita Gasiorowska, Jan Chojnacki, Cezary Chojnacki, Janusz Blasiak, Tomasz Poplawski |
|---|---|
| Přispěvatelé: | Department of Clinical Nutrition and Gastroenterological Diagnostics, Medical University of Lodz, 90-647 Lodz, Poland, Department of Molecular Genetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, Poland, Department of Gastroenterology, Medical University of Lodz, 90-647 Lodz, Poland |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Budesonide Lymphocytic colitis medicine.medical_specialty budesonide lcsh:Medicine Gastroenterology Article Pathogenesis 03 medical and health sciences 0302 clinical medicine Internal medicine medicine TPH1 enteroendocrine cells business.industry 5-Hydroxyindoleacetic acid lcsh:R General Medicine Tryptophan hydroxylase medicine.disease digestive system diseases serotonin lymphocytic colitis stomatognathic diseases 030104 developmental biology nervous system Intraepithelial lymphocyte 030211 gastroenterology & hepatology Serotonin business tryptophan hydroxylase 5-hydroxyindoleacetic acid medicine.drug |
| Zdroj: | Journal of Clinical Medicine, Vol 10, Iss 285, p 285 (2021) Journal of Clinical Medicine Volume 10 Issue 2 |
| ISSN: | 2077-0383 |
| Popis: | Lymphocytic colitis (LC) is a chronic inflammatory disease associated with watery diarrhea, abdominal pain, and colonic intraepithelial lymphocytosis. Serotonin (5-hydroxytryptamine, 5-HT) is reported to increase in certain colon diseases however, little is known regarding its metabolism in LC. In the present work, the level of 5-HT in serum and the number of enteroendocrine cells (EECs) as well as the expression of the 5-HT rate-limiting enzyme tryptophan hydroxylase 1 (TPH1) in colonic biopsies and urine 5-hydroxyindoeoacetic acid (5-HIAA) were determined in 36 LC patients that were treated with budesonide and 32 healthy controls. The 5-HT serum and 5-HIAA urine levels were measured using ELISA, the EEC number was determined immunohistochemically, and the colonic TPH1 mRNA expression was determined using RT-PCR. The levels of 5-HT and 5-HIAA and the number of EECs were higher in LC patients than in the controls, and positive correlations were observed between the 5-HT and 5-HIAA levels, 5-HT and EEC number, TPH1 mRNA and EEC number, as well as the severity of disease symptoms and 5-HIAA. Budesonide decreased the levels of 5-HT, 5-HIAA, and TPH1 expression and the number of EECs to values that did not differ from those for controls. In conclusion, the serotonin metabolism may be important for LC pathogenesis, and the urinary level of 5-HIAA may be considered as a non-invasive marker of this disease activity. |
| Databáze: | OpenAIRE |
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