Platelets disrupt vasculogenic mimicry by cancer cells
| Autor: | Emma J. Thompson, Carmela Martini, Emma C. Josefsson, Stephanie R. Hyslop, Brian Dale, Claudine S. Bonder, Anthony E. Woods, Lisa M. Ebert, Michaelia P. Cockshell |
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| Přispěvatelé: | Martini, Carmela, Thompson, Emma J, Hyslop, Stephanie R, Cockshell, Michaelia P, Dale, Brian J, Ebert, Lisa M, Woods, Anthony E, Josefsson, Emma C, Bonder, Claudine S |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Blood Platelets Male Angiogenesis lcsh:Medicine Apoptosis Breast Neoplasms Article Metastasis Neovascularization 03 medical and health sciences Mice 0302 clinical medicine Cell Line Tumor Neoplasms medicine Animals Platelet Vasculogenic mimicry Neoplasm Metastasis lcsh:Science Melanoma Mice Inbred BALB C Multidisciplinary Aspirin Neovascularization Pathologic business.industry lcsh:R Cancer medicine.disease tumour vasculature Endothelial stem cell 030104 developmental biology Mechanisms of disease 030220 oncology & carcinogenesis Cancer cell platelets Cancer research cancer cells lcsh:Q Female medicine.symptom business Neoplasm Transplantation Tumour angiogenesis |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-18 (2020) |
| Popis: | Tumour vasculature supports the growth and progression of solid cancers with both angiogenesis (endothelial cell proliferation) and vasculogenic mimicry (VM, the formation of vascular structures by cancer cells themselves) predictors of poor patient outcomes. Increased circulating platelet counts also predict poor outcome for cancer patients but the influence of platelets on tumour vasculature is incompletely understood. Herein, we show with in vitro assays that platelets did not influence angiogenesis but did actively inhibit VM formation by cancer cell lines. Both platelet sized beads and the releasates from platelets were partially effective at inhibiting VM formation suggesting that direct contact maximises the effect. Platelets also promoted cancer cell invasion in vitro. B16F10 melanomas in Bcl-xPlt20/Plt20 thrombocytopenic mice showed a higher content of VM than their wildtype counterparts while angiogenesis did not differ. In a xenograft mouse model of breast cancer with low-dose aspirin to inactivate the platelets, the burden of MDA-MB-231-LM2 breast cancer cells was reduced and the gene expression profile of the cancer cells was altered; but no effect on tumour vasculature was observed. Taken together, this study provides new insights into the action of platelets on VM formation and their involvement in cancer progression. |
| Databáze: | OpenAIRE |
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