Magnetic Barcoded Hydrogel Microparticles for Multiplexed Detection
| Autor: | Patrick S. Doyle, Stephen C. Chapin, Ki Wan Bong |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Materials science
Base Sequence Field (physics) Microfluidics Nucleic Acid Hybridization Hydrogels Nanotechnology Surfaces and Interfaces equipment and supplies Condensed Matter Physics Multiplexing Article Magnetic field Magnetics Dipole Nucleic Acids Self-healing hydrogels Electrochemistry General Materials Science DNA Probes Anisotropy human activities Lithography Spectroscopy |
| Zdroj: | Langmuir. 26:8008-8014 |
| ISSN: | 1520-5827 0743-7463 |
| DOI: | 10.1021/la904903g |
| Popis: | Magnetic polymer particles have been used in a wide variety of applications ranging from targeting and separation to diagnostics and imaging. Current synthesis methods have limited these particles to spherical or deformations of spherical morphologies. In this paper, we report the use of stop flow lithography to produce magnetic hydrogel microparticles with a graphical code region, a probe region, and a magnetic tail region. These anisotropic multifunctional magnetic polymer particles are an enhanced version of previously synthesized "barcoded" particles (Science, 2007, 315, 1393-1396) developed for the sensitive and rapid multiplexed sensing of nucleic acids. The newly added magnetic region has acquired dipole moments in the presence of weak homogeneous magnetic fields, allowing the particles to align along the applied field direction. The novel magnetic properties have led to practical applications in the efficient orientation and separation of the barcoded microparticles during biological assays without disrupting detection capabilities. |
| Databáze: | OpenAIRE |
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