Micro-stratified architectures based on successive stacking of alginate gel layers and poly(l-lysine)–hyaluronic acid multilayer films aimed at tissue engineering

Autor: Pierre Schaaf, Gero Decher, Fouzia Boulmedais, Nadia Benkirane-Jessel, Bernard Senger, Armelle Chassepot, Hajare Mjahed, Patrick Netter, Jean-Claude Voegel, Pierre Gillet, Claudine Porcel
Rok vydání: 2008
Předmět:
Zdroj: Soft Matter. 4:1422
ISSN: 1744-6848
1744-683X
DOI: 10.1039/b801428k
Popis: A micro-stratified 3D scaffold was designed by successive stacking of alginate gel layers (AGLs) and poly(l-lysine)-hyaluronic acid (PLL-HA) multilayer films. AGLs are obtained by complexation of alginate by Ca2+ ions. Alginate solutions are first sprayed onto a solid substrate inclined such that the excess of solution be removed by natural drainage. A CaCl2 solution is then either sprayed onto the substrate or the alginate covered substrate is dipped into a CaCl2 solution. The spraying of the CaCl2 solution leads to micro-porous AGLs, whereas the dipping in a CaCl2 aqueous solution leads to a more homogeneous gel layer without porosity. The second process also allows the formation of AGLs with a controlled thickness. With the goal of stacking different AGLs and PLL-HA films, the influence of a PLL-HA precursor film on the formation of AGLs is firstly investigated. It is found that when an alginate solution is sprayed on a PLL-HA multilayer built in the presence of CaCl2, the multilayer plays the role of reservoir of Ca2+ ions and of PLL chains, which both diffuse out of the multilayer film and complex alginate chains. This leads to the formation of a "pre-alginate gel". When this film is further dipped in the CaCl2 solution, an additional AGL forms, which is, however, free of PLL chains. Finally after the build-up of a PLL-HA film on the top of AGL, we succeeded in designing micro-stratified 3D scaffolds constituted by alternating strata of AGLs and PLL-HA films. This micro-stratified gel provides a new scaffold design with a perfectly controlled build-up: AGL aims to be a 3D scaffold for cell culture, and the PLL-HA multilayers should act as reservoirs for biologically active molecules.
Databáze: OpenAIRE
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