Transposition of minisatellite-like DNA in Chironomus midges
| Autor: | Angela Rohwedder, Thomas Hankeln, Erwin R. Schmidt, Bettina Weich |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Molecular Sequence Data Molecular evidence Genes Insect Subspecies DNA Satellite Genome Chironomidae Transposition (music) chemistry.chemical_compound Species Specificity Sequence Homology Nucleic Acid Genetics Animals Cloning Molecular Repeated sequence Molecular Biology In Situ Hybridization Repetitive Sequences Nucleic Acid integumentary system biology Base Sequence food and beverages General Medicine biology.organism_classification Minisatellite chemistry DNA Transposable Elements lipids (amino acids peptides and proteins) Chironomus Female DNA Biotechnology |
| Zdroj: | Genome. 37(4) |
| ISSN: | 0831-2796 |
| Popis: | Cla elements are a family of tandem repetitive DNA sequences present in the genome of several Chironomus species. Interspersed clusters of Cla elements are widely distributed all over the chromosomes in C. thummi thummi, while they seem to be limited to the centromeric regions in the closely related subspecies C. t. piger. Here we present molecular evidence that this differential distribution is due to a transposition of Cla elements during evolution of the C. t. thummi genome. We have cloned a "filled" integration site (containing a Cla element cluster) from C. t. thummi and the corresponding "empty" genomic site from C. t. piger and other related species. The comparison shows that tandem repetitive elements may be mobilized together with flanking DNA.Key words: minisatellites, transposition, Chironomus. |
| Databáze: | OpenAIRE |
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