Microsatellite DNA markers applied to detection of multiple paternity in Caiman latirostris in Santa Fe, Argentina

Autor: Patricia Susana Amavet, Esteban Rosso, Rosa Markariani, Carlos Ignacio Piña
Rok vydání: 2008
Předmět:
Zdroj: Journal of experimental zoology. Part A, Ecological genetics and physiology. 309(10)
ISSN: 1932-5223
Popis: Detecting multiple paternity in wild populations of the broad-snouted caiman (Caiman latirostris) has important implications for conservation efforts. We have applied microsatellite markers to examine genetic variation in C. latirostris and also have provided the first data concerning detection of multiple paternity in wild populations of this species. Blood samples from four nest-guarding C. latirostris females and their hatchlings were obtained from Santa Fe Province, Argentina. Amplified products were analyzed by electrophoresis on 10% polyacrylamide gels and visualized with silver staining. Four out of the eight markers tested reliably amplified and yielded useful data. Using polyacrylamide gels with silver staining provides high enough resolution to obtain individual genotypes. In order to assess the presence or absence of more than two parents in each nest, we used the single locus Minimum Method, and applied Cervus 3.0 and Gerud 2.0 software in parentage analyses. Our results indicate more than one father in at least two families. This behavior could be the consequence of high habitat variability in the area where our population was sampled. The ability to understand mating systems is important for maintaining viable populations of exploited taxa like C. latirostris. Fil: Amavet, Patricia Susana. Universidad Autonoma de Entre Rios; Argentina. Proyecto Yacaré; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias; Argentina Fil: Rosso, Esteban. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias; Argentina Fil: Markariani, Rosa. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias; Argentina Fil: Piña, Carlos Ignacio. Proyecto Yacare; Argentina. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina
Databáze: OpenAIRE