Prospective Monitoring Reveals Dynamic Levels of T Cell Immunity to Mycobacterium Tuberculosis in HIV Infected Individuals
| Autor: | Mona Pillay, Bruce D. Walker, Victoria O. Kasprowicz, Manjeetha Jaggernath, Thumbi Ndung'u, Jessica E. Mitchell, Pamla Govender, Anne Kasmar, Shivan Chetty, Paul Klenerman |
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| Rok vydání: | 2012 |
| Předmět: |
Bacterial Diseases
Viral Diseases Enzyme-Linked Immunospot Assay Time Factors T-Lymphocytes lcsh:Medicine HIV Infections Chemokine CXCL9 Limit of Detection Prospective Studies lcsh:Science Immune Response Multidisciplinary biology Coinfection Reverse Transcriptase Polymerase Chain Reaction ELISPOT Viral Load Infectious Diseases Medicine Viral load Research Article Tuberculosis Infectious Disease Control Immune Cells Immunology Microbiology Peripheral blood mononuclear cell Mycobacterium Mycobacterium tuberculosis Immune system Diagnostic Medicine Virology medicine Humans Biology lcsh:R HIV Reproducibility of Results biology.organism_classification medicine.disease CD4 Lymphocyte Count Chemokine CXCL10 Co-Infections Immunologic Techniques Clinical Immunology lcsh:Q |
| Zdroj: | PLoS ONE, Vol 7, Iss 6, p e37920 (2012) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0037920 |
| Popis: | Monitoring of latent Mycobacterium tuberculosis infection may prevent disease. We tested an ESAT-6 and CFP-10-specific IFN-(Gamma) Elispot assay (RD1-Elispot) on 163 HIV-infected individuals living in a TB-endemic setting. An RD1-Elispot was performed every 3 months for a period of 3-21 months. 62% of RD1-Elispot negative individuals were positive by cultured Elispot. Fluctuations in T cell response were observed with rates of change ranging from -150 to +153 spot-forming cells (SFC)/200,000 PBMC in a 3-month period. To validate these responses we used an RD1-specific real time quantitative PCR assay for monokine-induced by IFN-(Gamma)(MIG) and IFN-(Gamma) inducible protein-10 (IP10) (MIG: r=0.6527, p=0.0114; IP-10: r=0.6967, p=0.0056; IP-10+MIG: r=0.7055, p=0.0048). During follow-up 30 individuals were placed on ARVs and 4 progressed to active TB. Fluctuations in SFC did not correlate with CD4 count, viral load, treatment initiation, or progression to active TB. The RD1-Elispot appears to have limited value in this setting. |
| Databáze: | OpenAIRE |
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