Whole Mount In Situ Hybridization of Mid‐Gestation Mouse Embryos
| Autor: | Annemiek Beverdam |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Embryonic Development
Gene Expression In situ hybridization Biology medicine.disease_cause Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pregnancy medicine Animals Digoxigenin Gene In Situ Hybridization 030304 developmental biology 0303 health sciences Mutation Embryogenesis RNA Riboprobe Embryo RNA Probes General Medicine Cell biology chemistry embryonic structures Female 030217 neurology & neurosurgery |
| Zdroj: | Current Protocols in Mouse Biology. 10 |
| ISSN: | 2161-2617 |
| Popis: | In situ hybridization is a powerful technique that allows the visualization of specific RNA species in biological samples in exquisite detail. It has been particularly well explored in the field of developmental genetics. The spatial and temporal patterns of RNA expression provide us with critical information on likely gene function during embryonic development, and often inform the decision on whether to attempt further gene manipulation approaches. Furthermore, once a mouse strain with altered gene function has been created, in situ hybridization is a critical tool for revealing how the development of embryos with the mutation differs from that of wild-type embryos, and thus infer the function of the altered gene. Here, a well-tested protocol used to visualize RNA expression in whole-mount mid-gestation mouse embryos ranging from 8.5 to 14.5 days post-coitum (dpc) is described. © 2020 Wiley Periodicals LLC. Basic Protocol 1: RNA probe synthesis Alternate Protocol: Preparation of DNA template by PCR Basic Protocol 2: Embryo dissection Basic Protocol 3: Whole mount in situ hybridization Support Protocol: Generation of embryo powder. |
| Databáze: | OpenAIRE |
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