Are adjunctive markers useful in routine cervical cancer screening? Application of p16(INK4a) and HPV-PCR on ThinPrep samples with histological follow-up
| Autor: | Peter Horal, B. T. Andersen, M. Dohse, Magnus Lindh, Doris Schledermann, Walter Ryd, K. Bisgaard, B. Hoelund, Dorthe Ejersbo |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Histology Uterine Cervical Neoplasms Cervix Uteri Cervical intraepithelial neoplasia Cervical cancer screening Sensitivity and Specificity Pathology and Forensic Medicine law.invention law medicine Biomarkers Tumor Humans Cervical Intraepithelial Neoplasia Human papillomavirus Papillomaviridae neoplasms Polymerase chain reaction Cyclin-Dependent Kinase Inhibitor p16 Observer Variation Vaginal Smears integumentary system biology business.industry Reverse Transcriptase Polymerase Chain Reaction Histological Techniques Papillomavirus Infections Dna test General Medicine Middle Aged medicine.disease biology.organism_classification Uterine Cervical Dysplasia Immunohistochemistry Tumor Markers Biological DNA Viral Female business |
| Zdroj: | Schledermann, D, Andersen, B T, Bisgaard, K, Dohse, M, Ejersbo, D, Hoelund, B, Horal, P, Lindh, M & Ryd, W 2008, ' Are adjunctive markers useful in routine cervical cancer screening? Application of p16(INK4a) and HPV-PCR on ThinPrep samples with histological follow-up. ', Diagnostic Cytopathology, vol. 36, no. 7, pp. 453-9 . https://doi.org/10.1002/dc.20822 |
| ISSN: | 1097-0339 |
| DOI: | 10.1002/dc.20822 |
| Popis: | The objectives of the study were to evaluate 1) the diagnostic sensitivity and specificity of p16INK4a as a marker for high-grade cervical lesions, 2) the results of a real-time polymerase chain reaction detecting high-risk human papillomavirus, and 3) the interobserver variability of the p16INK4a interpretation. A total of 232 ThinPrep samples were stained for p16INK4a, and HPV-DNA PCR was performed on 107 specimens with inclusion of both benign and abnormal cytology. Histological follow-up information was collected. The diagnostic sensitivity of ASC+ with CIN2+ in histology as endpoint was 96% for p16INK4a and 100% for HR-HPV DNA PCR, and the diagnostic specificity was 41% and 27%, respectively. If p16INK4a had been used for triage of the ASC samples, then 18 patients (42%) could have been spared unnecessary follow-up procedures compared to six patients (21%) with the HR-HPV DNA test. Diagn. Cytopathol. 2008;36:453–459. © 2008 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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