Effects of cellulase and xylanase enzymes mixed with increasing doses of Salix babylonica extract on in vitro rumen gas production kinetics of a mixture of corn silage with concentrate

Autor: German Buendía-Rodríguez, Francisco J Peña Jiménez, Juan Carlos Vázquez Chagoyán, Maria Antonia Mariezcurrena Berasain, Mona M.M.Y. Elghandour, Miguel A. Rodríguez, María A. Cerrillo, Abdelfattah Z.M. Salem, Alberto Barbabosa Pliego
Rok vydání: 2015
Předmět:
Zdroj: Journal of Integrative Agriculture, Vol 14, Iss 1, Pp 131-139 (2015)
ISSN: 2095-3119
DOI: 10.1016/s2095-3119(13)60732-7
Popis: An in vitro gas production (GP) technique was used to investigate the effects of combining different doses of Salix babylonica extract (SB) with exogenous fibrolytic enzymes (EZ) based on xylanase (X) and cellulase (C), or their mixture (XC; 1:1 v/v) on in vitro fermentation characteristics of a total mixed ration of corn silage and concentrate mixture (50:50, w/w) as substrate. Four levels of SB (0, 0.6, 1.2 and 1.8 mL g−1 dry matter (DM)) and four supplemental styles of EZ (1 μL g−1 DM; control (no enzymes), X, C and XC (1:1, v/v) were used in a 4×4 factorial arrangement. In vitro GP (mL g−1 DM) were recorded at 2, 4, 6, 8, 10, 12, 24, 36, 48 and 72 h of incubation. After 72 h, the incubation process was stopped and supernatant pH was determined, and then filtered to determine dry matter degradability (DMD). Fermentation parameters, such as the 24 h gas yield (GY24), in vitro organic matter digestibility (OMD), metabolizable energy (ME), short chain fatty acid concentrations (SCFA), and microbial crude protein production (MCP) were also estimated. Results indicated that there was a SB×EZ interaction (Pon OMD, pH, ME, GY24, SCFA and MP. The combination of SB with EZ increased (P
Databáze: OpenAIRE
Externí odkaz: