A Capsule-Based Model for Immature Hard Tick Stages Infestation on Laboratory Mice
| Autor: | Lourdes Mateos-Hernández, Sabine Rakotobe, Alejandro Cabezas-Cruz, Baptiste Defaye, Ladislav Šimo |
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| Přispěvatelé: | Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Faculté de Pharmacie [Limoges], Université de Limoges (UNILIM), Sciences pour l'environnement (SPE), Centre National de la Recherche Scientifique (CNRS)-Université Pascal Paoli (UPP), DIM One Health - Région Île-de-France (Acronym of the project: NeuroPaTick), École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé |
| Rok vydání: | 2020 |
| Předmět: |
Ixodidae
General Chemical Engineering 030231 tropical medicine Zoology Tick medicine.disease_cause General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences 0302 clinical medicine Animals Laboratory parasitic diseases Infestation medicine Animals 030212 general & internal medicine Nymph Short duration General Immunology and Microbiology biology [SDV.BA]Life Sciences [q-bio]/Animal biology General Neuroscience Capsule Models Theoretical biology.organism_classification Blood feeding Hard ticks |
| Zdroj: | Journal of visualized experiments : JoVE Journal of visualized experiments : JoVE, JoVE, 2020, ⟨10.3791/61430⟩ jove.com/video/61430 |
| ISSN: | 1940-087X |
| DOI: | 10.3791/61430 |
| Popis: | International audience; Ticks are obligatory blood feeding parasites at all stages of development (except eggs) and are recognized as vectors of various pathogens. The use of mouse models in tick research is critical for understanding their biology and tick-host-pathogen interactions. Here we demonstrate a non-laborious technique for the feeding of immature stages of hard ticks on laboratory mice. The benefit of the method is its simplicity, short duration, and the ability to monitor or collect ticks at different time points of an experiment. In addition, the technique allows attachment of two individual capsules on the same mouse, which is beneficial for a variety of experiments where two different groups of ticks are required to feed on the same animal. The non-irritating and flexible capsule is made from easily accessible materials and minimizes the discomfort of the experimental animals. Furthermore, euthanasia is not necessary, mice recover completely after the experiment and are available for re-use. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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