Basidiochrome – A Novel Siderophore of the Orchidaceous Mycorrhizal Fungi Ceratobasidium and Rhizoctonia spp
| Autor: | Petra Hentschel, Klaus Albert, Dietmar G. Schmid, V. Passler, Kurt Haselwandter, S. Reiter, Graeme J. Nicholson, Günther Winkelmann |
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| Rok vydání: | 2006 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Siderophore Ceratobasidium Siderophores Tripeptide Hydroxamic Acids Rhizoctonia Peptides Cyclic Gas Chromatography-Mass Spectrometry General Biochemistry Genetics and Molecular Biology Biomaterials Mycorrhizae Spectroscopy Fourier Transform Infrared Botany medicine Mycorrhiza Chromatography High Pressure Liquid biology Basidiomycota Metals and Alloys Plant physiology Chromatography Ion Exchange biology.organism_classification Ferric General Agricultural and Biological Sciences Two-dimensional nuclear magnetic resonance spectroscopy medicine.drug |
| Zdroj: | BioMetals. 19:335-343 |
| ISSN: | 1572-8773 0966-0844 |
| DOI: | 10.1007/s10534-006-6986-x |
| Popis: | A novel trishydroxamate siderophore, named basidiochrome, was isolated as the principal siderophore from low-iron culture filtrates of Ceratobasidium and Rhizoctonia species which are known as mycorrhizal fungi associated with orchid roots. Ion-exchange chromatography and preparative HPLC yielded a pure compound which contained two components according to GC-MS analysis: L: -N(5)-hydroxy-ornithine and 3-methyl-2-cis-pentenedioic acid (3-methyl-cis-glutaconic acid). FTICR-ESI-MS of both the iron-free and ferric form indicated an elemental composition of C(33)H(47)N(6)O(16)Fe (MW = 839) for the ferric form of basidiochrome. The connectivity was further elucidated by 2D-NMR techniques (HSQC, HMBC, COSY, NOESY) indicating that basidiochrome is a novel linear tripeptide consisting of three L: -N(5)-hydroxy-ornithines each linked to 3-methyl-2-cis-pentenedioic acid residues. |
| Databáze: | OpenAIRE |
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