Preferential interaction of human immunodeficiency virus reverse transcriptase with two regions of primer tRNALys as evidenced by footprinting studies and inhibition with synthetic oligoribonucleotides
| Autor: | Philip J. Barr, Marie-Line Andreola, Karin Musier-Forsyth, Leila Sarih-Cottin, Simon Litvak, Bruno Bordier |
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| Rok vydání: | 1992 |
| Předmět: |
Nuclease
Oligoribonucleotides Base Sequence biology RNase P Molecular Sequence Data HIV RNA-Directed DNA Polymerase Ribonuclease Pancreatic Molecular biology Reverse transcriptase Kinetics chemistry.chemical_compound chemistry Structural Biology Transfer RNA biology.protein Nucleic Acid Conformation RNA Transfer Lys Pancreatic ribonuclease Dihydrouridine Primer (molecular biology) Molecular Biology |
| Zdroj: | Journal of Molecular Biology. 226:1-6 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/0022-2836(92)90117-3 |
| Popis: | Primer tRNA regions involved in the interactions between human immunodeficiency virus reverse transcriptase (HIV RT) and tRNA Lys were studied by digestion of primer with pancreatic ribonuclease in the presence or absence of HIV RT. The acceptor stem of tRNA Lys is not noticeably protected against nuclease action in the presence of HIV RT, while this enzyme clearly protects part of the anticodon and dihydrouridine loops of tRNA Lys . The acceptor stem of primer tRNA was digested by RNase A only in the presence of the retroviral enzyme, suggesting a partial destabilization of this region by the HIV RT. Synthetic oligoribonucleotides, corresponding to the anticodon and the dihydrouridine loops, inhibited strongly reverse transcription, confirming the strong interaction of these tRNA regions with the enzyme. |
| Databáze: | OpenAIRE |
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