Susceptibility of Microsporum canis arthrospores to a mixture of chemically defined essential oils: a perspective for environmental decontamination
| Autor: | Roberto Amerigo Papini, Silvia Giovanelli, Francesca Pisseri, Linda Mugnaini, Francesca Mancianti, Greta Profili, Luisa Pistelli, Simona Nardoni, Anna Maria Tortorano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
food.ingredient Antifungal Agents Foeniculum Microbial Sensitivity Tests medicine.disease_cause Cat Diseases General Biochemistry Genetics and Molecular Biology Microbiology Microsporum canis Minimum inhibitory concentration chemistry.chemical_compound food Tinea Zoonoses medicine Environmental Microbiology Oils Volatile Animals Humans Microsporum essential oils Decontamination Ecosystem Limonene antifungal activity biology Traditional medicine Litsea cubeba Spores Fungal biology.organism_classification chemistry Dermatophyte Pelargonium graveolens Cats Illicium verum Hair |
| Popis: | The zoophilic dermatophyte Microsporum canis has cats as natural reservoir, but it is able to infect a wide range of hosts, including humans, where different clinical features of the so-called ringworm dermatophytosis have been described. Human infections are increasingly been reported in Mediterranean countries. A reliable control program against M. canis infection in cats should include an antifungal treatment of both the infected animals and their living environment. In this article, a herbal mixture composed of chemically defined essential oils (EOs) of Litsea cubeba (1%), Illicium verum, Foeniculum vulgare, and Pelargonium graveolens (0.5% each) was formulated and its antifungal activity assessed against M. canis arthrospores which represent the infective environmental stage of M. canis. Single compounds present in higher amounts in the mixture were also separately tested in vitro. Litsea cubeba and P. graveolens EOs were most effective (minimum inhibitory concentration (MIC) 0.5%), followed by EOs of I. verum (MIC 2%) and F. vulgare (MIC 2.5%). Minimum fungicidal concentrations (MFC) values were 0.75% (L. cubeba), 1.5% (P. graveolens), 2.5% (I. verum) and 3% (F. vulgare). MIC and MFC values of the mixture were 0.25% and 0.5%, respectively. The daily spray of the mixture (200 μL) directly onto infected hairs inhibited fungal growth from the fourth day onwards. The compounds present in higher amounts exhibited variable antimycotic activity, with MIC values ranging from >10% (limonene) to 0.1% (geranial and neral). Thus, the mixture showed a good antifungal activity against arthrospores present in infected hairs. These results are promising for a further application of the mixture as an alternative tool or as an adjuvant in the environmental control of feline microsporosis. |
| Databáze: | OpenAIRE |
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