An alternative biocontrol agent of soil-borne phytopathogens: A new antifungal compound produced by a plant growth promoting bacterium isolated from North Algeria
| Autor: | Nassira Tabli, Sara Mirino, Ennio Cocca, Lorena Gratino, Elhafid Nabti, Gianna Palmieri, Bruna Agrillo, Rosarita Tatè, Marta Gogliettino |
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| Rok vydání: | 2019 |
| Předmět: |
Antifungal protein
Antifungal Agents Biological pest control Plant Development Microbial Sensitivity Tests Microbiology Conidium Conidia 03 medical and health sciences Pseudomonas protegens Solanum lycopersicum Plant Growth Regulators Biocontrol agent Pseudomonas Spore germination Soil Microbiology 030304 developmental biology 0303 health sciences Molecular mass biology 030306 microbiology fungi Alternaria Plants Spores Fungal biology.organism_classification Fungicide Stereomicroscopy and TEM analyses Biological Control Agents Algeria Rhizosphere Bacteria |
| Zdroj: | Microbiological research 221 (2019): 60–69. doi:10.1016/j.micres.2019.02.004 info:cnr-pdr/source/autori:Agrillo, Bruna; Mirino, Sara; Tate, Rosarita; Gratino, Lorena; Gogliettino, Marta; Cocca, Ennio; Tabli, Nassira; Nabti, Elhafid; Palmieri, Gianna/titolo:An alternative biocontrol agent of soil-borne phytopathogens: A new antifungal compound produced by a plant growth promoting bacterium isolated from North Algeria/doi:10.1016%2Fj.micres.2019.02.004/rivista:Microbiological research (Print)/anno:2019/pagina_da:60/pagina_a:69/intervallo_pagine:60–69/volume:221 |
| ISSN: | 0944-5013 |
| DOI: | 10.1016/j.micres.2019.02.004 |
| Popis: | Bacteria isolated from different environments can be exploited for biocontrol purposes by the identification of the molecules involved in the antifungal activity. The present study was aimed at investigating antifungal protein compounds purified from a previously identified plant growth promoting bacterium, Pseudomonas protegens N isolated from agricultural land in northern Algeria. Therefore, a novel protein was purified by chromatographic and ultrafiltration steps and its antifungal activity together with growth-inhibition mechanism was evaluated against different fungi by plate-based assays. In addition, stereomicroscopy and transmission electron microscopy (TEM) was performed to explore the inhibition activity of the compound on spore germination processes. The protein, showing a molecular mass of about 100 kDa under native conditions, was revealed to be in the surface-membrane fraction and displayed an efficient activity against a variety of phytopathogenic fungi, being Alternaria the best target towards which it exhibited a marked fungicidal action and inhibition of spore germination. Moreover, the compound was able to significantly decrease fungal infection on tomato fruits producing also morphological aberrations on conidia. The obtained results suggested that the isolated compound could represent a promising agent for eco-friendly management of plant pathogens in agriculture. |
| Databáze: | OpenAIRE |
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