Surface morphology and function of human pulmonary alveolar macrophages from smokers and non-smokers
| Autor: | H. Kohrogi, S. Horio, R Nishi, M. Sugimoto, Kazuyoshi Shimazu, Shukuro Araki, Moritaka Suga, Masayuki Ando |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Nitroblue tetrazolium medicine.medical_treatment Phagocytosis In Vitro Techniques Cell Adhesion medicine Humans Macrophage Membrane surface Saline business.industry Macrophages Nitroblue Tetrazolium Cell Membrane Smoking Respiratory disease Macrophage Activation beta-Galactosidase medicine.disease In vitro respiratory tract diseases Pulmonary Alveoli medicine.anatomical_structure Microscopy Electron Scanning behavior and behavior mechanisms Pulmonary alveolus Lysosomes business Oxidation-Reduction Research Article |
| Zdroj: | Thorax. 39:850-856 |
| ISSN: | 0040-6376 |
| DOI: | 10.1136/thx.39.11.850 |
| Popis: | Pulmonary alveolar macrophages were obtained by saline lavage from 23 healthy male volunteers--10 non-smokers and 13 cigarette smokers. Lavage produced three times as many alveolar macrophages in smokers than in non-smokers. When macrophages from smokers and from non-smokers were incubated in vitro, more cells from smokers adhered to glass, spread out, and showed enhanced nitroblue tetrazolium (NBT) reduction. The surface morphology of alveolar macrophages from smokers showed more with a plate like appearance and ridge like membrane surface, while the macrophages from non-smokers were predominantly spherical with ruffles. The proportions of cells which stained highly for beta galactosidase were 55% in smokers and 11% in non-smokers. Thus, in a resting state in vitro, alveolar macrophages from smokers were more active than those from non-smokers. When, however, macrophages from smokers and non-smokers were incubated with immunobeads and with opsonised or non-opsonised BCG, the phagocytic activity and stimulated NBT reduction of alveolar macrophages from smokers were similar to or somewhat less than those of non-smokers. |
| Databáze: | OpenAIRE |
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