| Autor: |
Janik, Karolina, Treda, Cezary, Wlodarczyk, Aneta, Peciak, Joanna, Rosiak, Kamila, Zieba, Jolanta, Grot, Dagmara, Rutkowska, Adrianna, Pawlowska, Roza, Och, Waldemar, Rieske, Piotr, Stoczynska-Fidelus, Ewelina |
| Rok vydání: |
2019 |
| DOI: |
10.6084/m9.figshare.9838490.v1 |
| Popis: |
Additional file 3: Table S3. Molecular changes during passages in each analyzed glioblastoma cell culture. Sanger sequencing for IDH1 codon 132 and TP53 exons 4–8; wt – wild type; amp – amplification; MLPA ratio was interpreted as normal copy number (0.7–1.3); deletion of one allele (0.35–0.65), deletion of both alleles (0), gain of one allele (e.g. trisomy) (1.35–1.55), gain of more than one allele (1.6–2.2), while the value in range of 0.1–0.3 was considered to result from the analysis of heterogeneous materialin which deletion was detected; ±SD. Results of Real-time PCR for EGFRWT and EGFRvIII expression were analyzed as previously described [23]. Results of Real-time PCR for DNA copy number were analyzed as described in ‘Materials & Methods’ section. (DOCX 63 kb) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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