Bioinformatic, phylogenetic and chemical analysis of the UV-absorbing compounds scytonemin and mycosporine-like amino acids from the microbial mat communities of Shark Bay, Australia
| Autor: | Hon Lun Wong, Brendan P. Burns, Luděk Sehnal, Brett A. Neilan, Jason N. Woodhouse, Heng Tai Liew, Russel Pickford, Paul M. D'Agostino |
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| Přispěvatelé: | School of Biological Sciences |
| Rok vydání: | 2018 |
| Předmět: |
Cyanobacteria
Indoles Ultraviolet Rays Glycine Context (language use) Scytonemin Photosynthesis Microbiology 03 medical and health sciences chemistry.chemical_compound Phylogenetic Phenols Phylogenetics Tandem Mass Spectrometry Botany 14. Life underwater Microbial mat Amino Acids Ecology Evolution Behavior and Systematics Phylogeny 030304 developmental biology Bioinformatic 0303 health sciences biology Phylogenetic tree 030306 microbiology Microbiota Australia Computational Biology biology.organism_classification Science::Biological sciences [DRNTU] chemistry Bays Bay |
| Zdroj: | Environmental microbiology. 21(2) |
| ISSN: | 1462-2920 |
| Popis: | Shark Bay, Western Australia is a World Heritage area with extensive microbial mats and stromatolites. Microbial communities that comprise these mats have developed a range of mitigation strategies against changing levels of photosynthetically active and ultraviolet radiation, including the ability to biosynthesise the UV-absorbing natural products scytonemin and mycosporine-like amino acids (MAAs). To this end, the distribution of photoprotective pigments within Shark Bay microbial mats was delineated in the present study. This involved amplicon sequencing of bacterial 16S rDNA from communities at the surface and subsurface in three distinct mat types (smooth, pustular and tufted), and correlating this data with the chemical and molecular distribution of scytonemin and MAAs. Employing UV spectroscopy and MS/MS fragmentation, mycosporine-glycine, asterina and an unknown MAA were identified based on typical fragmentation patterns. Marker genes for scytonemin and MAA production (scyC and mysC) were amplified from microbial mat DNA and placed into phylogenetic context against a broad screen throughout 363 cyanobacterial genomes. Results indicate that occurrence of UV screening compounds is associated with the upper layer of Shark Bay microbial mats, and the occurrence of scytonemin is closely dependent on the abundance of cyanobacteria. |
| Databáze: | OpenAIRE |
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