Modification of Human Parotid Saliva Proteins by Oral Streptococcus sanguis
| Autor: | Q. T. Smith, Sun-Jin Choih, Charles F. Schachtele |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
0301 basic medicine
Time Factors Proline Proteolysis medicine.medical_treatment medicine.disease_cause Microbiology 03 medical and health sciences 0302 clinical medicine Serine medicine Humans Parotid Gland Protease Inhibitors Sulfhydryl Compounds Salivary Proteins and Peptides General Dentistry Polyacrylamide gel electrophoresis chemistry.chemical_classification Protease medicine.diagnostic_test biology Streptococcus 030206 dentistry biology.organism_classification Protease inhibitor (biology) 030104 developmental biology Enzyme Biochemistry chemistry Electrophoresis Polyacrylamide Gel Streptococcus sanguis Bacteria Peptide Hydrolases medicine.drug |
| Zdroj: | Journal of Dental Research. 58:516-524 |
| ISSN: | 1544-0591 0022-0345 |
| Popis: | Polyacrylamide gel electrophoresis was used to analyze the effect of Streptococcus sanguis on the anionic proteins in human parotid gland saliva. Cell-free culture supernatants and washed-cell preparations from 23 strains of S. sanguis caused marked modification of various salivary proteins. Control studies showed that the alterations in protein profiles by the bacteria were not due to attachment of protein to the cells. Protease inhibitors were used to confirm that proteolysis by distinct enzymatic activities was responsible for most of the modifications. There was no discernible relationship between the degradation patterns and the various immunologic or genetic groups of S. sanguis. Proteins which contained high concentrations of proline were extensively degraded by S. sanguis. This effect could be blocked with the protease inhibitor phenylmethylsulphonyl fluoride. |
| Databáze: | OpenAIRE |
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