Receptor binding assay for the detection of paralytic shellfish poisoning toxins: comparison to the mouse bioassay and applicability under regulatory use
| Autor: | S. Kusum Perera, Gregg W. Langlois, Raphael M. Kudela, Shiyamalie R. Ruberu, Clive Kittredge, Melisa Masuda |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Health Toxicology and Mutagenesis mouse bioassay Early detection Biology Toxicology 01 natural sciences saxitoxins 03 medical and health sciences chemistry.chemical_compound Mice Food Sciences Mouse bioassay Paralytic shellfish poisoning medicine Animals Shellfish Poisoning comparability receptor binding assay Shellfish Saxitoxin Chromatography 010401 analytical chemistry Significant difference Public Health Environmental and Occupational Health General Chemistry General Medicine Mussel porcine Alert level medicine.disease 0104 chemical sciences 030104 developmental biology chemistry Biological Assay Marine Toxins Food Science |
| Zdroj: | Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, vol 35, iss 1 Ruberu, SR; Langlois, GW; Masuda, M; Kittredge, C; Perera, SK; & Kudela, RM. (2018). Receptor binding assay for the detection of paralytic shellfish poisoning toxins: comparison to the mouse bioassay and applicability under regulatory use. FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 35(1), 144-158. doi: 10.1080/19440049.2017.1369584. UC Santa Cruz: Retrieved from: http://www.escholarship.org/uc/item/091057bg |
| ISSN: | 1944-0057 |
| DOI: | 10.1080/19440049.2017.1369584. |
| Popis: | The receptor-binding assay (RBA) method for the detection of paralytic shellfish poisoning (PSP) toxins was evaluated for its overall performance in comparison with the mouse bioassay (MBA). An initial study to evaluate the effects of filtering shellfish extracts prior to running the RBA indicated no significant difference between filtered and unfiltered extracts on the determined saxitoxin (STX) concentrations. Next, we tested the RBA assay on 295 naturally contaminated mussel tissue samples, ranging in concentrations from 320 µg STX equiv. kg-1 to 13,000 µg STX equiv. kg-1 by MBA. An overall trend was observed with the RBA giving higher results (256 µg STX equiv. kg-1 on average) than the MBA; however, at low concentrations (< 500 µg STX equiv. kg-1) the RBA results were marginally lower. A third study was conducted using spiked mussel tissue analysed by three independent laboratories, two of which performed the RBA and one the MBA. This multi-laboratory study again showed the RBA to give higher results than the MBA; however, it also revealed that STX determination was accurate by the RBA, unlike the MBA. To optimise the assay for efficient usage under regulatory practice, three suggestions have been made: the use of an initial screening plate to separate those samples that exceed the alert level; use of rapid PSP test kits in the field and in the laboratory for screening negative samples and for early detection of toxicity; and use of an alternate commercially available porcine membrane in place of the laboratory-prepared rat membrane homogenate. The large number of samples analysed and the diversity of the tests conducted in this study further support the RBA as an affordable rapid method for STX detection that is also free of the routine sacrifice of live animals. |
| Databáze: | OpenAIRE |
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