Involvement of S6 kinase and p38 mitogen activated protein kinase pathways in strain-induced alignment and proliferation of bovine aortic smooth muscle cells
| Autor: | Wei Li, Quanhai Chen, Ira Mills, Bauer E. Sumpio |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
MAP Kinase Signaling System
Pyridines Physiology p38 mitogen-activated protein kinases Myocytes Smooth Muscle Clinical Biochemistry Cell Biology p38 Mitogen-Activated Protein Kinases Muscle Smooth Vascular Smooth muscle medicine Animals Enzyme Inhibitors Aorta Cells Cultured Sirolimus Strain (chemistry) Kinase Ribosomal Protein S6 Kinases Imidazoles Cell Biology Phenotype Cell biology DNA-Binding Proteins Coupling (electronics) medicine.anatomical_structure Regional Blood Flow cardiovascular system Cattle Stress Mechanical Mitogen-Activated Protein Kinases Cell Division Immunosuppressive Agents Intracellular Transcription Factors |
| Zdroj: | Journal of Cellular Physiology. 195:202-209 |
| ISSN: | 1097-4652 0021-9541 |
| DOI: | 10.1002/jcp.10230 |
| Popis: | Bovine aortic smooth muscle cell (SMC) phenotype can be altered by physical forces. This has been demonstrated by cyclic strain-induced changes in proliferation and alignment. However, the intracellular coupling pathways remain ill defined. In the present study, we examined whether the p38 and S6 kinase pathway were involved in the mitogenic and morphological changes seen in SMCs exposed to cyclic strain. We seeded bovine aortic SMCs on silastic membranes that were deformed with 150-mmHg vacuum. Cyclic strain induced both alignment and proliferation of SMCs. SB202190, a specific inhibitor of p38, hindered SMC alignment, but not proliferation. Rapamycin, a specific inhibitor of the mTOR-S6 kinase pathway, attenuated strain-induced proliferation, but not alignment. Peak activation of p38 and S6 kinase was 351 ± 76.9% at 5 min and 363 ± 56.2% at 60 min compared with static control, respectively (P |
| Databáze: | OpenAIRE |
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