Rap1, Canoe and Mbt cooperate with Bazooka to promote zonula adherens assembly in the fly photoreceptor
| Autor: | Franck Pichaud, Rhian F Walther, Mubarik Burki, Noelia Pinal, Clare Rogerson |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
endocrine system animal structures Telomere-Binding Proteins Morphogenesis AFDN Zonula adherens Context (language use) CDC42 Biology Shelterin Complex Adherens junction 03 medical and health sciences Bazooka Animals Drosophila Proteins Guanine Nucleotide Exchange Factors Small GTPase Photoreceptor Cells Epithelial polarity Rap1 Photoreceptor Intracellular Signaling Peptides and Proteins Cell Biology Adherens Junctions Cadherins Par3 Cell biology enzymes and coenzymes (carbohydrates) 030104 developmental biology Drosophila melanogaster Pak4 Protein Kinases Zonula adherens assembly Protein Binding Research Article |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Journal of Cell Science |
| Popis: | In Drosophila epithelial cells, apical exclusion of Bazooka (the Drosophila Par3 protein) defines the position of the zonula adherens (ZA), which demarcates the apical and lateral membrane and allows cells to assemble into sheets. Here, we show that the small GTPase Rap1, its effector Canoe (Cno) and the Cdc42 effector kinase Mushroom bodies tiny (Mbt), converge in regulating epithelial morphogenesis by coupling stabilization of the adherens junction (AJ) protein E-Cadherin and Bazooka retention at the ZA. Furthermore, our results show that the localization of Rap1, Cno and Mbt at the ZA is interdependent, indicating that their functions during ZA morphogenesis are interlinked. In this context, we find the Rap1- GEF Dizzy is enriched at the ZA and our results suggest that it promotes Rap1 activity during ZA morphogenesis. Altogether, we propose the Dizzy, Rap1 and Cno pathway and Mbt converge in regulating the interface between Bazooka and AJ material to promote ZA morphogenesis. RFW, MB and NP was funded by an MRC grant to FP (award code MC_UU_12018/3) |
| Databáze: | OpenAIRE |
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