Blood–Brain Barrier Permeability of Green Tea Catechin Metabolites and their Neuritogenic Activity in Human Neuroblastoma SH‐SY5Y Cells

Autor: Fumio Nanjo, Akira Minami, Yoriyuki Nakamura, Akiko Takagaki, Kazuaki Iguchi, Monira Pervin, Aimi Nakagawa, Hara Aya, Keiko Unno
Rok vydání: 2017
Předmět:
Zdroj: Molecular Nutrition & Food Research. 61:1700294
ISSN: 1613-4133
1613-4125
DOI: 10.1002/mnfr.201700294
Popis: cope To understand the mechanism by which green tea lowers the risk of dementia, focus was placed on the metabolites of epigallocatechin gallate (EGCG), the most abundant catechin in green tea. Much of orally ingested EGCG is hydrolyzed to epigallocatechin (EGC) and gallic acid. In rats, EGC is then metabolized mainly to 5-(3’, 5’-dihydroxyphenyl)-γ-valerolactone (EGC-M5) and its conjugated forms, which are distributed to various tissues. Therefore, we examined the permeability of these metabolites into the blood-brain barrier (BBB), and nerve cell proliferation/differentiation in vitro. Methods and results The permeability of EGC-M5, glucuronide and the sulfate of EGC-M5, pyrogallol, as well as its glucuronide into the BBB were examined using a BBB model kit. Each brain- and blood-side sample was subjected to liquid chromatography tandem-mass spectrometry analysis. BBB permeability (%, in 0.5 h) was 1.9-3.7 %. In human neuroblastoma SH-SY5Y cells, neurite length was significantly prolonged by EGC-M5, and the number of neurites was increased significantly by all metabolites examined. Conclusion The permeability of EGC-M5 and its conjugated forms into the BBB suggests that they reached the brain parenchyma. In addition, the ability of EGC-M5 to affect nerve cell proliferation and neuritogenesis suggests that EGC-M5 may promote neurogenesis in the brain. This article is protected by copyright. All rights reserved
Databáze: OpenAIRE
Externí odkaz: