Edible pectin film added with peptides from jackfruit leaves obtained by high-hydrostatic pressure and pepsin hydrolysis

Autor: Julio César Barros-Castillo, Montserrat Calderón-Santoyo, Maricarmen Iñiguez-Moreno, Juan Arturo Ragazzo-Sánchez, Carolina Calderón-Chiu, Irving Antonio Brion-Espinoza
Jazyk: angličtina
Rok vydání: 2021
Předmět:
food.ingredient
Pectin
Hydrostatic pressure
education
Amino acid standards (AA-S-18)
Pepsin (EC 3.4.23.1)
Bradford reagent serine standard (PubChem CID: 5951)
Pancreatin (EC 232-468-9)
Food processing and manufacture
Analytical Chemistry
Artocarpus
Hydrolysis
food
Hydrochloric acid (HCl PubChem CID: 313)
Pepsin
N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA >97% PubChem CID: 2724275)
Spore germination
TX341-641
Food science
Article(s) from the Special Issue on Novel proteins and peptides for human consumption: sources
chemistry and function by Dr. González-Córdova and Dr. Sáyago-Ayerdi

Water content
Mycelium
Amino acid profile
biology
Nutrition. Foods and food supply
Chemistry
L-Norleucine (Nor ≥98% PubChem CID: 21236)
Acetonitrile (PubChem CID: 6342 HPLC grade ≥99.8%)
food and beverages
Low-methoxyl (LM) pectin (29–33% Food grade E-440)
TP368-456
Jackfruit leaf
biology.organism_classification
Colletotrichum gloeosporioides
Postharvest protection
Biocomposite film
biology.protein
cardiovascular system
Food Science
circulatory and respiratory physiology
Glycerol (PubChem CID: 753) sodium hydroxide (NaOH PubChem CID: 14798)
Zdroj: Food Chemistry: X
Food Chemistry: X, Vol 12, Iss, Pp 100170-(2021)
ISSN: 2590-1575
Popis: Highlights • Fresh leaves of jackfruit are a suitable source of protein. • Six essential amino acids were identified in peptides obtained by pepsin (Pep-P). • Pep-P from jackfruit leaves inhibited Colletotrichum gloeosporioides. • Pep-P improved the physicochemical and microbiological properties of pectin films.
Jackfruit (Artocarpus heterophyllus Lam.) is an evergreen tree that produces a high waste of leaves. This study evaluated the obtention of peptides from jackfruit leaves using pancreatin and pepsin, their antifungal activity, and their effect on pectin films. The protein content was 7.64 ± 0.12 g/100 g of jackfruit fresh leaves. Pancreatin produced a higher yield than pepsin in the obtention of peptides (p ≤ 0.05). However, peptides obtained after 2 h by pepsin hydrolysis (Pep-P) had six essential amino acids and inhibited > 99% of mycelial growth and spore germination of Colletotrichum gloeosporioides. Pectin films with Pep-P showed a slight brown color, lower thickness, water vapor permeability, and moisture content, as well as higher thermal stability and better inhibition properties against C. gloeosporioides than pectin films without Pep-P (p ≤ 0.05). Pectin films added with Pep-P from jackfruit leaf could be a green alternative to anthracnose control in tropical fruits.
Databáze: OpenAIRE