Assessing the utilization of high-resolution 2-field HLA typing in solid organ transplantation
| Autor: | Michael T. Mignogno, Allison Gasiewski, Dimitri S. Monos, Jamie L. Duke, Timothy Mosbruger, Ethan Kentzel, Ryan Morlen, Edward C. Frackelton, Larissa Slavich, S. Heron, Steven S. Geier, Deborah Ferriola, Yanping Huang, Anh Dinh, Hilary Mehler, Olga A. Timofeeva, Carolina Kneib |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Adolescent High resolution Human leukocyte antigen symbols.namesake HLA Antigens Risk Factors Antibody identification Immunogenetics Immunology and Allergy Medicine Humans Pharmacology (medical) Typing Child Retrospective Studies Sanger sequencing Transplantation business.industry Histocompatibility Testing Patient Selection High-Throughput Nucleotide Sequencing Infant Organ Transplantation Sequence Analysis DNA University hospital Prognosis Tissue Donors Histocompatibility Child Preschool Immunology symbols Female Solid organ transplantation business Follow-Up Studies |
| Zdroj: | American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 19(7) |
| ISSN: | 1600-6143 |
| Popis: | HLA typing in solid organ transplantation (SOT) is necessary for determining HLA-matching status between donor-recipient pairs and assessing patients' anti-HLA antibody profiles. Histocompatibility has traditionally been evaluated based on serologically defined HLA antigens. The evolution of HLA typing and antibody identification technologies, however, has revealed many limitations with using serologic equivalents for assessing compatibility in SOT. The significant improvements to HLA typing introduced by next-generation sequencing (NGS) require an assessment of the impact of this technology on SOT. We have assessed the role of high-resolution 2-field HLA typing (HR-2F) in SOT by retrospectively evaluating NGS-typed pre- and post-SOT cases. HR-2F typing was highly instructive or necessary in 41% (156/385) of the cases. Several pre- and posttransplant scenarios were identified as being better served by HR-2F typing. Five different categories are presented with specific case examples. The experience of another center (Temple University Hospital) is also included, whereby 21% of the cases required HR-2F typing by Sanger sequencing, as supported by other legacy methods, to properly address posttransplant anti-HLA antibody issues. |
| Databáze: | OpenAIRE |
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