Protective mechanism of 1-methylhydantoin against lung injury induced by paraquat poisoning

Autor: Jinyi Lan, Lei Ren, Yifan Wei, Lina Gao, Bo Liu, Annan Chen
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
Anti-Inflammatory Agents
Phenylalanine
Pharmacology
Pathology and Laboratory Medicine
Biochemistry
chemistry.chemical_compound
Mice
0302 clinical medicine
Paraquat
Drug Metabolism
Malondialdehyde
Medicine and Health Sciences
Metabolites
Immune Response
Lung
Protein Metabolism
chemistry.chemical_classification
Multidisciplinary
biology
Poisoning
Lung Injury
Enzymes
Chemistry
Dismutases
030220 oncology & carcinogenesis
Physical Sciences
Medicine
Metabolic Pathways
Research Article
Nicotine
Science
Immunology
Acute Lung Injury
Pulmonary Edema
Lung injury
Superoxide dismutase
03 medical and health sciences
Signs and Symptoms
Alkaloids
Diagnostic Medicine
Lactate dehydrogenase
Animals
Metabolomics
Pharmacokinetics
Inflammation
Reactive oxygen species
Nicotinamide
L-Lactate Dehydrogenase
Superoxide Dismutase
Tumor Necrosis Factor-alpha
Hydantoins
Chemical Compounds
Biology and Life Sciences
Proteins
030104 developmental biology
Metabolism
chemistry
biology.protein
Enzymology
Zdroj: PLoS ONE
PLoS ONE, Vol 14, Iss 9, p e0222521 (2019)
ISSN: 1932-6203
Popis: Paraquat (PQ), one of the most widely used herbicides worldwide, causes severe toxic effects in humans and animals. 1-methylhydantoin (MH) is an active ingredient of Ranae Oviductus, which has broad pharmacological activities, e.g., eliminating reactive oxygen species and inhibiting inflammation. This study investigated the effects of MH on lung injury induced by PQ. A PQ poisoning model was established by intragastric infusion of PQ (25 mg/kg), and the control group was simultaneously gavaged with the same dose of saline. The MH group was intraperitoneally injected with 100 mg/kg once per day after intragastric infusion of PQ (25 mg/kg) for five consecutive days. All animals were sacrificed on the sixth day, and the lung tissues were dissected for metabolomics analysis. The lactate dehydrogenase (LDH) activity, superoxide dismutase (SOD) activity, TNF-α and malondialdehyde (MDA) content were determined according to the instructions of the detection kit. Compared with that in the control group, the content of LDH, TNF-α and MDA in the lung tissue of the PQ group was significantly higher, and the activity of SOD in the lung tissue was significantly lower (all p 0.05). There were significant differences in MDA and TNF-α content between the PQ group and MH group (all p
Databáze: OpenAIRE
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