Protective mechanism of 1-methylhydantoin against lung injury induced by paraquat poisoning
Autor: | Jinyi Lan, Lei Ren, Yifan Wei, Lina Gao, Bo Liu, Annan Chen |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Male Anti-Inflammatory Agents Phenylalanine Pharmacology Pathology and Laboratory Medicine Biochemistry chemistry.chemical_compound Mice 0302 clinical medicine Paraquat Drug Metabolism Malondialdehyde Medicine and Health Sciences Metabolites Immune Response Lung Protein Metabolism chemistry.chemical_classification Multidisciplinary biology Poisoning Lung Injury Enzymes Chemistry Dismutases 030220 oncology & carcinogenesis Physical Sciences Medicine Metabolic Pathways Research Article Nicotine Science Immunology Acute Lung Injury Pulmonary Edema Lung injury Superoxide dismutase 03 medical and health sciences Signs and Symptoms Alkaloids Diagnostic Medicine Lactate dehydrogenase Animals Metabolomics Pharmacokinetics Inflammation Reactive oxygen species Nicotinamide L-Lactate Dehydrogenase Superoxide Dismutase Tumor Necrosis Factor-alpha Hydantoins Chemical Compounds Biology and Life Sciences Proteins 030104 developmental biology Metabolism chemistry biology.protein Enzymology |
Zdroj: | PLoS ONE PLoS ONE, Vol 14, Iss 9, p e0222521 (2019) |
ISSN: | 1932-6203 |
Popis: | Paraquat (PQ), one of the most widely used herbicides worldwide, causes severe toxic effects in humans and animals. 1-methylhydantoin (MH) is an active ingredient of Ranae Oviductus, which has broad pharmacological activities, e.g., eliminating reactive oxygen species and inhibiting inflammation. This study investigated the effects of MH on lung injury induced by PQ. A PQ poisoning model was established by intragastric infusion of PQ (25 mg/kg), and the control group was simultaneously gavaged with the same dose of saline. The MH group was intraperitoneally injected with 100 mg/kg once per day after intragastric infusion of PQ (25 mg/kg) for five consecutive days. All animals were sacrificed on the sixth day, and the lung tissues were dissected for metabolomics analysis. The lactate dehydrogenase (LDH) activity, superoxide dismutase (SOD) activity, TNF-α and malondialdehyde (MDA) content were determined according to the instructions of the detection kit. Compared with that in the control group, the content of LDH, TNF-α and MDA in the lung tissue of the PQ group was significantly higher, and the activity of SOD in the lung tissue was significantly lower (all p 0.05). There were significant differences in MDA and TNF-α content between the PQ group and MH group (all p |
Databáze: | OpenAIRE |
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