Dynamics of extended-spectrum cephalosporin-resistant Escherichia coli in pig farms: A longitudinal study
| Autor: | Markus Hilty, Anne Oppliger, Suzanne Aebi, Julia Moor, Gudrun Overesch, Susanne Rickli, Nadezda Mostacci |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Veterinary medicine Swine animal diseases Cephalosporin Prevalence Liquid manure Adult Aged Aged 80 and over Animals Anti-Bacterial Agents/therapeutic use Cephalosporin Resistance/drug effects Cephalosporins/therapeutic use Escherichia coli/drug effects Escherichia coli/genetics Escherichia coli Infections/drug therapy Escherichia coli Infections/epidemiology Escherichia coli Infections/veterinary Farmers/statistics & numerical data Farms/statistics & numerical data Feces/microbiology Female Genome-Wide Association Study Humans Longitudinal Studies Middle Aged Occupational Exposure/statistics & numerical data Switzerland/epidemiology Antimicrobial resistance Clonal spread Extended-spectrum beta-lactamase Fattening pig Occupational exposure Piglet medicine.disease_cause Feces 0302 clinical medicine Pig farming Pharmacology (medical) 030212 general & internal medicine 610 Medicine & health Escherichia coli Infections Farmers 630 Agriculture Cephalosporin Resistance General Medicine Anti-Bacterial Agents Infectious Diseases Switzerland Microbiology (medical) Farms medicine.drug_class 030106 microbiology Biology 03 medical and health sciences Antibiotic resistance Occupational Exposure medicine Escherichia coli Weaning Cephalosporins Carriage 570 Life sciences biology |
| Zdroj: | International journal of antimicrobial agents, vol. 58, no. 3, pp. 106382 Moor, Julia; Aebi, Susanne; Rickli, Susanne; Mostacci, Nadezda; Overesch, Gudrun; Oppliger, Anne; Hilty, Markus (2021). Dynamics of extended-spectrum cephalosporin-resistant Escherichia coli in pig farms: A longitudinal study. International journal of antimicrobial agents, 58(3), p. 106382. Elsevier 10.1016/j.ijantimicag.2021.106382 |
| DOI: | 10.1016/j.ijantimicag.2021.106382 |
| Popis: | OBJECTIVES Point prevalence estimates of extended-spectrum cephalosporin-resistant Escherichia coli (ESC-R-Ec) are important surveillance measures but may not uncover the ESC-R-Ec dynamics within pig farms. A longitudinal study was therefore performed by sampling individual pigs, pig farmers and the environment. METHODS On average, 30 (range 10-46) piglets of 31 Swiss farms were sampled during the suckling, weaning and fattening stages (n= 2437 samples). In addition, stool from pig farmers and environmental samples were obtained and metadata collected by questionnaires. ESC-R-Ec was identified by routine culture, and clonal relationships and resistance genes were derived from whole genome sequencing data. RESULTS Working on pig farms was not associated with an increased prevalence of ESC-R-Ec in humans. ESC-R-Ec prevalence significantly decreased from 6.2% to 3.9% and 1.8% for the suckling, weaned and fattening pigs, respectively (P < 0.001). Within the 57 ESC-R-positive suckling piglets, persisting carriage was detected in 25 animals at two consecutive time points and one animal at three consecutive time points. Clonal spread (n=7 farms, 22.6%) and horizontal gene transfer (n=1 farm, 3%) within pigs but not between humans and animals was detected. Liquid manure (n=10 samples, 16.7%) was identified as the major environmental reservoir of ESC-R-Ec in the pig farm environment. CONCLUSIONS Pig farming practices like all-in-all-out systems, but not antimicrobial usage, were associated with reduced risk of ESC-R-Ec at the farm level. As carriage duration is normally short within the individual pigs, the risk of recolonisation and clonal spread of ESC-R-Ec might be reduced by applying appropriate decontamination strategies. |
| Databáze: | OpenAIRE |
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